| Intracellular ATP delivery using highly fusogenic liposomes. | |
MedLine Citation:
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PMID: 20072895 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Healthy cells must maintain a high content of adenosine triphosphate (ATP) because almost all energy-requiring processes in cells are driven, either directly or indirectly, by hydrolysis of ATP. During ischemia or hypoxia, reduced blood flow or disturbed oxygen supply results in the disrupted balance of energy production and utilization, and depletion of high-energy phosphates is the fundamental cause of cell damage. Direct intravenous infusion of high-energy phosphates, such as adenosine triphosphate (ATP), has not produced a consistent result because strongly charged molecules like ATP normally cannot pass the cell membrane in sufficient quantities to satisfy tissue metabolic requirements. Furthermore, the half-life of free ATP in blood circulation is very short, limiting its efficacy as a bioenergetic substrate. We have developed a new technique for intracellular delivery of high-energy phosphate into normal or ischemic cells by using specially formulated, highly fusogenic, unilamellar lipid vesicles that contain magnesium-ATP. In vitro studies indicated a rapid fusion with the endothelial cells, protection of endothelial cells, and cardiomyocytes during ischemia. In vivo studies have shown enhanced full-thickness skin wound healing in various animal models. This technique has the potential to reduce or eliminate many detrimental effects caused by ischemia or hypoxia. |
Authors:
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Sufan Chien |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Methods in molecular biology (Clifton, N.J.) Volume: 605 ISSN: 1940-6029 ISO Abbreviation: Methods Mol. Biol. Publication Date: 2010 |
Date Detail:
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Created Date: 2010-01-14 Completed Date: 2010-04-07 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9214969 Medline TA: Methods Mol Biol Country: United States |
Other Details:
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Languages: eng Pagination: 377-91 Citation Subset: IM |
Affiliation:
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Department of Surgery, University of Louisville, Louisville, KY, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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administration & dosage*,
pharmacokinetics*,
therapeutic use Animals Cell Hypoxia / drug effects Cell Membrane Permeability Cells, Cultured Endothelial Cells / cytology, drug effects Humans Ischemia / drug therapy Liposomes / chemistry, pharmacokinetics* Myocytes, Cardiac / cytology, drug effects Rabbits Rats Rats, Sprague-Dawley Skin / metabolism Wound Healing / drug effects* |
| Grant Support | |
ID/Acronym/Agency:
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AR52984/AR/NIAMS NIH HHS; DK74566/DK/NIDDK NIH HHS; HL64186/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Liposomes; 56-65-5/Adenosine Triphosphate |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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