Document Detail


Intracarotid infusion of leukotriene C4 selectively increases blood-brain barrier permeability after focal ischemia in rats.
MedLine Citation:
PMID:  1675639     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Intracarotid infusions of leukotriene C4 (LTC4) were used to open selectively the blood-brain barrier (BBB) in ischemic tissue after middle cerebral artery (MCA) occlusion in rats. BBB permeability was determined by quantitative autoradiography using [14C]aminoisobutyric acid. Seventy-two hours after MCA occlusion, LTC4 (4 micrograms total dose) infused into the carotid artery ipsilateral to the MCA occlusion selectively increased the unidirectional transfer constant for permeability Ki approximately threefold within core ischemic tissue and tissue adjacent ot the ischemic core. No effect on BBB permeability was seen within nonischemic brain tissue or in ischemic tissue after only 24 h after MCA occlusion. gamma-Glutamyl transpeptidase (gamma-GTP) activity was decreased in capillaries in ischemic tissue at 48 and 72 h after infarction, compared to high gamma-GTP in normal brain capillaries and moderate gamma-GTP in capillaries in the ischemic tissue at 24 h after infarction. These findings suggest that normal brain capillaries resist the vasogenic effects of LTC4. In contrast, LTC4 increases permeability in capillaries of ischemic tissue, where gamma-GTP is decreased. gamma-Glutamyl transpeptidase, an enzyme that inactivates LTC4 to LTD4 and LTE4 to LTF4, may act as an "enzymatic barrier" in normal brain capillaries to leukotrienes.
Authors:
T Baba; K L Black; K Ikezaki; K N Chen; D P Becker
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism     Volume:  11     ISSN:  0271-678X     ISO Abbreviation:  J. Cereb. Blood Flow Metab.     Publication Date:  1991 Jul 
Date Detail:
Created Date:  1991-07-25     Completed Date:  1991-07-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8112566     Medline TA:  J Cereb Blood Flow Metab     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  638-43     Citation Subset:  IM    
Affiliation:
Division of Neurosurgery, UCLA Medical Center 90024.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood-Brain Barrier / drug effects*
Brain / blood supply
Capillaries / enzymology
Capillary Permeability
Carotid Arteries
Cerebral Arteries
Cerebral Infarction / etiology,  pathology
Constriction
Female
Infusions, Intra-Arterial
Ischemic Attack, Transient / complications,  pathology,  physiopathology*
Rats
Rats, Inbred Strains
SRS-A / administration & dosage,  pharmacology*
gamma-Glutamyltransferase / metabolism
Grant Support
ID/Acronym/Agency:
1R29NS26523-01/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/SRS-A; EC 2.3.2.2/gamma-Glutamyltransferase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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