Document Detail

Intra-uterine tissue engineering of full-thickness skin defects in a fetal sheep model.
MedLine Citation:
PMID:  20170954     Owner:  NLM     Status:  MEDLINE    
In spina bifida the neural tube fails to close during the embryonic period and it is thought that prolonged exposure of the unprotected spinal cord to the amniotic fluid during pregnancy causes additional neural damage. Intra-uterine repair might protect the neural tissue from exposure to amniotic fluid and might reduce additional neural damage. Biodegradable collagen scaffolds may be useful in case of fetal therapy for spina bifida, but biochemical properties need to be studied. The aim of this study was to investigate whether biodegradable collagen scaffolds can be used to treat full-thickness fetal skin defects. We hypothesized that the pro-angiogenic growth factors VEGF and FGF2 would enhance vascularization, epidermialization and lead to improved wound healing. To investigate the effect of these two growth factors, a fetal sheep model for skin defects was used. Compared to wounds treated with bare collagen scaffolds, wounds treated with growth factor-loaded scaffolds showed excessive formation of capillaries and less myofibroblasts were present in these wounds, leading to less contraction. This study has demonstrated that collagen scaffolds can be used to treat fetal skin defects and that the combination of collagen scaffolds with VEGF and FGF2 had a beneficial effect on wound healing.
Nynke A Hosper; Alex J Eggink; Luc A J Roelofs; Rene M H Wijnen; Marja J A van Luyn; Ruud A Bank; Martin C Harmsen; Paul J Geutjes; Willeke F Daamen; Toin H van Kuppevelt; Dorien M Tiemessen; Egbert Oosterwijk; Jane J Crevels; Willeke A M Blokx; Fred K Lotgering; Paul P van den Berg; Wout F J Feitz
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-02-18
Journal Detail:
Title:  Biomaterials     Volume:  31     ISSN:  1878-5905     ISO Abbreviation:  Biomaterials     Publication Date:  2010 May 
Date Detail:
Created Date:  2010-03-15     Completed Date:  2010-06-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8100316     Medline TA:  Biomaterials     Country:  England    
Other Details:
Languages:  eng     Pagination:  3910-9     Citation Subset:  IM    
Copyright Information:
Copyright 2010 Elsevier Ltd. All rights reserved.
University Medical Center Groningen, University of Groningen, Department of Pathology and Medical Biology, Groningen, The Netherlands.
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MeSH Terms
Epithelium / pathology
Fetus / pathology*,  surgery
Fibroblasts / pathology
Microscopy, Electron, Scanning
Models, Animal*
Neovascularization, Pathologic
Sheep / surgery
Skin / blood supply,  pathology*
Tissue Engineering / methods*
Tissue Scaffolds
Wound Healing

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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