Document Detail

Intra-portal injection of 400- microm microcapsules in a large-animal model.
MedLine Citation:
PMID:  12819871     Owner:  NLM     Status:  MEDLINE    
To date, encapsulated grafts have usually been implanted in the peritoneal cavity. This site is, however, not ideal, mainly because of its low blood supply. We have investigated the feasibility of intra-portal injection of (400 microm) microcapsules in the pig. Ten-thousand microcapsules per kilogram body weight were injected into six Large White pigs. Portal pressure, various biological tests, portographies and liver histology were recorded before and at various time points after injection. As a result, portal pressure increased after injection (15+/-2.3 vs 8.7+/-1.7 mmHg) but remained within an acceptable range (<20 mmHg) and returned to normal values at 3 months (8.5+/-3.7 mmHg). During the 3-month follow up, liver function and liver tests remained stable. Portographies showed a homogenous implantation of the capsule, with the portal flow always directed to the liver. At histological examination after 3 months the capsules demonstrated various degrees of fibrosis. We can thus conclude that these results demonstrate that intra-portal injection of microcapsules is feasible in a large-animal model. Hemodynamic, biological and radiological results are similar to those observed in clinical free-islet transplantation.
Christian Toso; José Oberholzer; Ion Ceausoglu; Frédéric Ris; Bertrand Rochat; Annemie Rehor; Pascal Bucher; Christine Wandrey; Ute Schuldt; Jacques Belenger; Domenico Bosco; Philippe Morel; David Hunkeler
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-03-20
Journal Detail:
Title:  Transplant international : official journal of the European Society for Organ Transplantation     Volume:  16     ISSN:  0934-0874     ISO Abbreviation:  Transpl. Int.     Publication Date:  2003 Jun 
Date Detail:
Created Date:  2003-06-23     Completed Date:  2004-03-02     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8908516     Medline TA:  Transpl Int     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  405-10     Citation Subset:  IM    
Division d'Investigations Chirurgicales, Clinique de Chirurgie Digestive et Unité de Transplantation, Hôpital Universitaire, 24 Rue Micheli-du-Crest, 1211 Geneva, Switzerland.
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MeSH Terms
Capsules / administration & dosage*
Feasibility Studies
Follow-Up Studies
Injections, Intravenous
Liver / pathology
Portal Pressure
Portal Vein*
Reg. No./Substance:

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