| Intra- and interlaboratory reproducibility of an ELISA serological test for Lyme disease. | |
| | |
MedLine Citation:
|
PMID: 22416209 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
|
OBJECTIVES: Lyme disease has been increasingly diagnosed throughout North America since the late 1970s. The clinical diagnosis and epidemiological monitoring of Lyme disease are aided by serological testing for the etiological agent, Borrelia burgdorferi. Numerous authorities have questioned the reproducibility of these serological tests. This study assessed the intra- and interlaboratory reproducibility of an elisa used to aid in the diagnosis of Lyme disease. METHODS: Twenty-seven sera from cases and noncases were tested by three laboratories. Two of the laboratories repeated the tests once. These testings were part of the 1991 quality control assessment of provincial laboratories carried out by the Laboratory Centre for Disease Control (lcdc), Ottawa. RESULTS: The mean weighted kappa statistics were 0.87 for interlaboratory comparisons and 0.89 for intralaboratory comparisons. CONCLUSIONS: Overall, the elisa assessed in this study demonstrated good to excellent intra- and interlaboratory reproducibility in the lcdc 1991 quality control assessment when the data were assessed in the categorical scale using the weighted kappa statistic. Generalization of these findings to clinical laboratory settings must be done with caution. |
| | |
Authors:
|
M C Tammemagi; J W Frank; M Leblanc; H Artsob |
Related Documents
:
|
22375569 - A pilot test of the latin active hip hop intervention to increase physical activity amo... 22686339 - Dissolution testing of orally disintegrating tablets. 22857959 - Reliability of metatarsus adductus angle and correlation with hallux valgus. 22573409 - Laboratory identification of lupus anticoagulants. 22315859 - Diagnostic dilemma of dengue fever: how much reliability are there in rapidity. 22394029 - Reliability of the dynamic gait index (finnish version) in individuals with neurologica... 17430079 - In search of the holy grail: comparison of antibody screening methods. 3156739 - 99mtc-plasmin test in deep vein thrombosis of the leg. 6779619 - Ergonovine testing to detect spontaneous remissions of variant angina during long-term ... |
Publication Detail:
|
Type: Journal Article |
Journal Detail:
|
Title: The Canadian journal of infectious diseases = Journal canadien des maladies infectieuses Volume: 6 ISSN: 1180-2332 ISO Abbreviation: Can J Infect Dis Publication Date: 1995 Mar |
Date Detail:
|
Created Date: 2012-03-14 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 9425856 Medline TA: Can J Infect Dis Country: Canada |
Other Details:
|
Languages: eng Pagination: 90-5 Citation Subset: - |
Affiliation:
|
Graduate Department of Community Health, and Department of Preventative Medicine and Biostatistics, University of Toronto, Toronto, and Laboratory Centre for Disease Control, Ottawa, Ontario. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Activity of vancomycin, teicoplanin and cephalosporins against penicillin-susceptible and penicillin...
Next Document: Eradication of methicillin-resistant Staphylococcus aureus from the lower respiratory tract of patie...