Document Detail

Intra- and interlaboratory reproducibility of an ELISA serological test for Lyme disease.
MedLine Citation:
PMID:  22416209     Owner:  NLM     Status:  PubMed-not-MEDLINE    
OBJECTIVES: Lyme disease has been increasingly diagnosed throughout North America since the late 1970s. The clinical diagnosis and epidemiological monitoring of Lyme disease are aided by serological testing for the etiological agent, Borrelia burgdorferi. Numerous authorities have questioned the reproducibility of these serological tests. This study assessed the intra- and interlaboratory reproducibility of an elisa used to aid in the diagnosis of Lyme disease.
METHODS: Twenty-seven sera from cases and noncases were tested by three laboratories. Two of the laboratories repeated the tests once. These testings were part of the 1991 quality control assessment of provincial laboratories carried out by the Laboratory Centre for Disease Control (lcdc), Ottawa.
RESULTS: The mean weighted kappa statistics were 0.87 for interlaboratory comparisons and 0.89 for intralaboratory comparisons.
CONCLUSIONS: Overall, the elisa assessed in this study demonstrated good to excellent intra- and interlaboratory reproducibility in the lcdc 1991 quality control assessment when the data were assessed in the categorical scale using the weighted kappa statistic. Generalization of these findings to clinical laboratory settings must be done with caution.
M C Tammemagi; J W Frank; M Leblanc; H Artsob
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The Canadian journal of infectious diseases = Journal canadien des maladies infectieuses     Volume:  6     ISSN:  1180-2332     ISO Abbreviation:  Can J Infect Dis     Publication Date:  1995 Mar 
Date Detail:
Created Date:  2012-03-14     Completed Date:  2012-08-23     Revised Date:  2013-05-29    
Medline Journal Info:
Nlm Unique ID:  9425856     Medline TA:  Can J Infect Dis     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  90-5     Citation Subset:  -    
Graduate Department of Community Health, and Department of Preventative Medicine and Biostatistics, University of Toronto, Toronto, and Laboratory Centre for Disease Control, Ottawa, Ontario.
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