Document Detail

Intra- and extracellular measurement of reactive oxygen species produced during heat stress in diaphragm muscle.
MedLine Citation:
PMID:  11003586     Owner:  NLM     Status:  MEDLINE    
Skeletal muscles are exposed to increased temperatures during intense exercise, particularly in high environmental temperatures. We hypothesized that heat may directly stimulate the reactive oxygen species (ROS) formation in diaphragm (one kind of skeletal muscle) and thus potentially play a role in contractile and metabolic activity. Laser scan confocal microscopy was used to study the conversion of hydroethidine (a probe for intracellular ROS) to ethidium (ET) in mouse diaphragm. During a 30-min period, heat (42 degrees C) increased ET fluorescence by 24 +/- 4%, whereas in control (37 degrees C), fluorescence decreased by 8 +/- 1% compared with baseline (P < 0.001). The superoxide scavenger Tiron (10 mM) abolished the rise in intracellular fluorescence, whereas extracellular superoxide dismutase (SOD; 5,000 U/ml) had no significant effect. Reduction of oxidized cytochrome c was used to detect extracellular ROS in rat diaphragm. After 45 min, 53 +/- 7 nmol cytochrome c. g dry wt(-1). ml(-1) were reduced in heat compared with 22 +/- 13 nmol. g(-1). ml(-1) in controls (P < 0.001). SOD decreased cytochrome c reduction in heat to control levels. The results suggest that heat stress stimulates intracellular and extracellular superoxide production, which may contribute to the physiological responses to severe exercise or the pathology of heat shock.
L Zuo; F L Christofi; V P Wright; C Y Liu; A J Merola; L J Berliner; T L Clanton
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  279     ISSN:  0363-6143     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2000 Oct 
Date Detail:
Created Date:  2000-10-12     Completed Date:  2000-10-30     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  C1058-66     Citation Subset:  IM    
Department of Internal Medicine, Pulmonary and Critical Care Medicine, Ohio State University, Columbus, Ohio 43210, USA.
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MeSH Terms
1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt / pharmacology
Antioxidants / pharmacology
Catalase / metabolism
Cell Nucleus / metabolism
Cytochrome c Group / metabolism
Cytoplasm / metabolism
Diaphragm / metabolism
Extracellular Space / metabolism*
Heat Stress Disorders / metabolism*
Intracellular Fluid / metabolism*
Muscle Contraction / physiology
Rats, Sprague-Dawley
Reactive Oxygen Species / metabolism*
Respiratory Muscles / metabolism*
Superoxide Dismutase / metabolism,  pharmacology
Grant Support
Reg. No./Substance:
0/Antioxidants; 0/Cytochrome c Group; 0/Reactive Oxygen Species; 149-45-1/1,2-Dihydroxybenzene-3,5-Disulfonic Acid Disodium Salt; 3546-21-2/Ethidium; EC; EC Dismutase

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