Document Detail

Intra-cage air change rate on forced-air-ventilated micro-isolation system--environment within cages: carbon dioxide and oxygen concentration.
MedLine Citation:
PMID:  9353632     Owner:  NLM     Status:  MEDLINE    
Recently, a forced-air-ventilated micro-isolation system (FVMIS) has been recognized to accurately maintain microenvironmental conditions inside cages, but the details of the relationship between the concentrations of carbon dioxide (CO2) and oxygen (O2) and the air change rate inside the cages have never been reported. In this study, the proper intra-cage air change rate was examined based on the CO2 concentration and O2 concentration inside the cages measured by changing the ventilation volume inside the closed cages of the FVMIS while housing animals. In the experiments, three 8-week-old Wistar strain male rats weighing 303 g on average were housed in each FVMIS cage (capacity: 0.0223 m3), and the temperature, relative humidity, CO2 concentration and O2 concentration were measured when the air change rate inside the cages was varied from 10 air changes per hour (ACH) to 120 ACH. It proved that the CO2 concentration in the FVMIS cages decreased uniformly with the increase in the air change rate. As a result, 60 ACH was required to maintain the CO2 concentration level inside the FVMIS cages equivalent to or less than that in the conventional housing. Otherwise, when based on the O2 concentration, 50 ACH was required. In consideration of these results and others based on ventilation, airflow, temperature and the ammonia concentration reported previously, we concluded that the proper air change rate inside the FVMIS cages should be approximately 60 ACH.
M Hasegawa; Y Kurabayashi; T Ishii; K Yoshida; N Uebayashi; N Sato; T Kurosawa
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Experimental animals / Japanese Association for Laboratory Animal Science     Volume:  46     ISSN:  1341-1357     ISO Abbreviation:  Exp. Anim.     Publication Date:  1997 Oct 
Date Detail:
Created Date:  1997-11-28     Completed Date:  1997-11-28     Revised Date:  2014-10-24    
Medline Journal Info:
Nlm Unique ID:  9604830     Medline TA:  Exp Anim     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  251-7     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Air / standards
Animals, Laboratory
Carbon Dioxide / analysis*
Housing, Animal*
Oxygen / analysis*
Rats, Wistar
Reg. No./Substance:
142M471B3J/Carbon Dioxide; S88TT14065/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Diabetes and accident insurance. A 3-year follow-up of 7,599 insured diabetic individuals.
Next Document:  Immunohistochemical study on type II collagen-induced arthritis in DBA/1J mice.