| Intestinal uptake of quercetin-3-glucoside in rats involves hydrolysis by lactase phlorizin hydrolase. | |
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MedLine Citation:
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PMID: 12612151 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Quercetin has antioxidant, anti-inflammatory, antiproliferative and anticarcinogenic properties. In plant foods, quercetin occurs mainly bound to various sugars via a beta-glycosidic link. We hypothesized that lactase phlorizin hydrolase (LPH), an enzyme at the brush border membrane of intestinal cells, is involved in the in vivo intestinal uptake of quercetin-sugars. To study this, we measured the appearance of quercetin metabolites in plasma and perfusate after perfusing the jejunum and ileum with 50 micro mol/L quercetin-3-glucoside in an in situ rat perfusion model. LPH was inhibited by the selective LPH inhibitor N-butyldeoxygalactonojirimycin (0, 0.5, 2 or 10 mmol/L) (n = 5 rats/group). Quercetin in plasma and perfusion buffer was determined by HPLC with CoulArray detection. Results are given as means +/- SEM. In the perfusion buffer, 13.8 +/- 0.7 micro mol/L quercetin-3-glucoside was hydrolyzed during intestinal passage. Co-perfusion with 0.5, 2 and 10 mmol/L N-butyldeoxygalactonojirimycin resulted in 38% (P < 0.05), 50% (P < 0.01) and 67% (P < 0.01) less hydrolysis, respectively. Plasma concentrations of quercetin in the corresponding groups were 36% (P = 0.12), 55% (P < 0.01) and 75% (P < 0.01) lower than in controls (1.23 +/- 0.22 micro mol/L). These data suggest that LPH is a major determinant of intestinal absorption of quercetin-3-glucoside in rats. |
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Authors:
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Aloys L A Sesink; Ilja C W Arts; Maria Faassen-Peters; Peter C H Hollman |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of nutrition Volume: 133 ISSN: 0022-3166 ISO Abbreviation: J. Nutr. Publication Date: 2003 Mar |
Date Detail:
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Created Date: 2003-03-03 Completed Date: 2003-04-11 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0404243 Medline TA: J Nutr Country: United States |
Other Details:
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Languages: eng Pagination: 773-6 Citation Subset: IM |
Affiliation:
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Department of Pharmacology and Toxicology, Nijmegen Center for Molecular Life Sciences, The Netherlands. a.sesink@ncmls.kun.nl |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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1-Deoxynojirimycin
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analogs & derivatives*,
pharmacology Animals Chromatography, High Pressure Liquid Enzyme Inhibitors / pharmacology Flavonols* Glycosylceramidase / antagonists & inhibitors, metabolism* Hydrolysis Ileum / enzymology Intestines / enzymology*, ultrastructure Jejunum / enzymology Male Microvilli / enzymology Quercetin / analogs & derivatives*, blood, metabolism* Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/Enzyme Inhibitors; 0/Flavonols; 0/N-butyldeoxygalactonojirimycin; 117-39-5/Quercetin; 19130-96-2/1-Deoxynojirimycin; 21637-25-2/isoquercitrin; 480-19-3/3-methylquercetin; EC 3.2.1.62/Glycosylceramidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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