| Intestinal protein and LPH synthesis in parenterally fed piglets receiving partial enteral nutrition and enteral insulinlike growth factor 1. | |
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MedLine Citation:
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PMID: 11568522 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Providing partial enteral nutrition (PEN) supplemented with insulinlike growth factor-1 (IGF-1) to parenterally fed piglets increases lactase-phlorizin hydrolase (LPH) activity, but not LPH mRNA. The current aim was to investigate potential mechanisms by which IGF-1 up-regulates LPH activity. METHODS: Newborn piglets (n = 15) received 100% parenteral nutrition (TPN), 80% parenteral nutrition + 20% parenteral nutrition (PEN), or PEN + IGF-1 (1.0 mg. kg-1. d-1) for 7 days. On day 7, [2H3]-leucine was intravenously administered to measure mucosal protein and brush border LPH (BB LPH) synthesis. RESULTS: Weight gain, nutrient intake, and jejunal weight and length were similar among the treatment groups. Partial enteral nutrition alone increased mucosal weight, villus width and cross-sectional area, LPH activity, mRNA expression, and high mannose LPH precursor (proLPHh) abundance compared with TPN (P<0.05). Insulinlike growth factor-1 further increased mucosal weight, LPH activity, and LPH activity per unit BB LPH approximately twofold over PEN alone (P < 0.05) but did not affect LPH mRNA or the abundance of proLPHh (one of the LPH isoforms) or mature LPH. Isotopic enrichment of [2H3]-leucine in plasma, mucosal protein, and LPH precursors, and the fractional and absolute synthesis rates of mucosal protein and LPH were similar among the treatment groups. Insulinlike growth factor-1 treatment increased total mucosal protein synthesis (60%, P < 0.05) but not LPH synthesis compared with the other two groups. CONCLUSIONS: Because IGF-1 did not affect the fractional synthesis rate of either mucosal protein or LPH, the authors suggest that enteral IGF-1 increases mucosal protein mass and LPH activity by suppressing mucosal proteolytic degradation. |
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Authors:
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Y K Park; M A Dudley; D G Burrin; S M Donovan |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of pediatric gastroenterology and nutrition Volume: 33 ISSN: 0277-2116 ISO Abbreviation: J. Pediatr. Gastroenterol. Nutr. Publication Date: 2001 Aug |
Date Detail:
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Created Date: 2001-09-24 Completed Date: 2001-10-25 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 8211545 Medline TA: J Pediatr Gastroenterol Nutr Country: United States |
Other Details:
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Languages: eng Pagination: 189-95 Citation Subset: IM |
Affiliation:
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Division of Nutritional Sciences, University of Illinois, Urbana 61801, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Enteral Nutrition* Glycosylceramidase / biosynthesis, metabolism* Insulin-Like Growth Factor I / administration & dosage, metabolism, pharmacology* Intestinal Mucosa / enzymology* Jejunum / enzymology Parenteral Nutrition Protein Biosynthesis* RNA, Messenger / analysis Swine Weight Gain |
| Grant Support | |
ID/Acronym/Agency:
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HD29264/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/RNA, Messenger; 67763-96-6/Insulin-Like Growth Factor I; EC 3.2.1.62/Glycosylceramidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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