Document Detail


Intestinal protein and LPH synthesis in parenterally fed piglets receiving partial enteral nutrition and enteral insulinlike growth factor 1.
MedLine Citation:
PMID:  11568522     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
BACKGROUND: Providing partial enteral nutrition (PEN) supplemented with insulinlike growth factor-1 (IGF-1) to parenterally fed piglets increases lactase-phlorizin hydrolase (LPH) activity, but not LPH mRNA. The current aim was to investigate potential mechanisms by which IGF-1 up-regulates LPH activity. METHODS: Newborn piglets (n = 15) received 100% parenteral nutrition (TPN), 80% parenteral nutrition + 20% parenteral nutrition (PEN), or PEN + IGF-1 (1.0 mg. kg-1. d-1) for 7 days. On day 7, [2H3]-leucine was intravenously administered to measure mucosal protein and brush border LPH (BB LPH) synthesis. RESULTS: Weight gain, nutrient intake, and jejunal weight and length were similar among the treatment groups. Partial enteral nutrition alone increased mucosal weight, villus width and cross-sectional area, LPH activity, mRNA expression, and high mannose LPH precursor (proLPHh) abundance compared with TPN (P<0.05). Insulinlike growth factor-1 further increased mucosal weight, LPH activity, and LPH activity per unit BB LPH approximately twofold over PEN alone (P < 0.05) but did not affect LPH mRNA or the abundance of proLPHh (one of the LPH isoforms) or mature LPH. Isotopic enrichment of [2H3]-leucine in plasma, mucosal protein, and LPH precursors, and the fractional and absolute synthesis rates of mucosal protein and LPH were similar among the treatment groups. Insulinlike growth factor-1 treatment increased total mucosal protein synthesis (60%, P < 0.05) but not LPH synthesis compared with the other two groups. CONCLUSIONS: Because IGF-1 did not affect the fractional synthesis rate of either mucosal protein or LPH, the authors suggest that enteral IGF-1 increases mucosal protein mass and LPH activity by suppressing mucosal proteolytic degradation.
Authors:
Y K Park; M A Dudley; D G Burrin; S M Donovan
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Journal of pediatric gastroenterology and nutrition     Volume:  33     ISSN:  0277-2116     ISO Abbreviation:  J. Pediatr. Gastroenterol. Nutr.     Publication Date:  2001 Aug 
Date Detail:
Created Date:  2001-09-24     Completed Date:  2001-10-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8211545     Medline TA:  J Pediatr Gastroenterol Nutr     Country:  United States    
Other Details:
Languages:  eng     Pagination:  189-95     Citation Subset:  IM    
Affiliation:
Division of Nutritional Sciences, University of Illinois, Urbana 61801, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Animals, Newborn
Enteral Nutrition*
Glycosylceramidase / biosynthesis,  metabolism*
Insulin-Like Growth Factor I / administration & dosage,  metabolism,  pharmacology*
Intestinal Mucosa / enzymology*
Jejunum / enzymology
Parenteral Nutrition
Protein Biosynthesis*
RNA, Messenger / analysis
Swine
Weight Gain
Grant Support
ID/Acronym/Agency:
HD29264/HD/NICHD NIH HHS
Chemical
Reg. No./Substance:
0/RNA, Messenger; 67763-96-6/Insulin-Like Growth Factor I; EC 3.2.1.62/Glycosylceramidase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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