| Intestinal polymeric immunoglobulin receptor is affected by type and route of nutrition. | |
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MedLine Citation:
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PMID: 17712142 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Secretory immunoglobulin A (SIgA) prevents adherence of pathogens at mucosal surfaces to prevent invasive infection. Polymeric immunoglobulin receptor (pIgR) is located on the basolateral surface of epithelial cells and binds dimeric immunoglobulin A (IgA) produced by plasma cells in the lamina propria. This IgA-pIgR complex is transported apically, where IgA is exocytosed as SIgA to the mucosal surface. Our prior work shows that mice fed intragastric (IG, an elemental diet model) and IV parenteral nutrition (PN) solution have reduced intestinal T and B cells, SIgA, and interleukin-4 (IL-4) compared with mice fed chow or a complex enteral diet (CED). Prior work also demonstrates a reduction in IgA transport to mucosal surfaces in IV PN-fed mice. Because IL-4 up-regulates pIgR production, this work studies the effects of these diets on intestinal pIgR. METHODS: Male Institute of Cancer Research (ICR) mice were randomized to chow (n = 11) with IV catheter, CED (n = 10) or IG PN (n = 11) via gastrostomy and IV PN (n = 12) for 5 days. CED and PN were isocaloric and isonitrogenous. Small intestine was harvested for pIgR and IL-4 assays after mucosal washing for IgA. IgA and IL-4 levels were analyzed by enzyme-linked immunosorbent assay and pIgR by Western blot. RESULTS: Small intestinal pIgR expression, IgA levels, and IL-4 levels decreased significantly in IV PN and IG PN groups. CONCLUSIONS: Lack of enteral stimulation affects multiple mechanisms responsible for decreased intestinal SIgA levels, including reduced T and B cells in the lamina propria, reduced Th-2 IgA-stimulating cytokines, and impaired expression of the IgA transport protein, pIgR. |
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Authors:
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Yoshifumi Sano; F Enrique Gomez; Woodae Kang; Jinggang Lan; Yoshinori Maeshima; Joshua L Hermsen; Chikara Ueno; Kenneth A Kudsk |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: JPEN. Journal of parenteral and enteral nutrition Volume: 31 ISSN: 0148-6071 ISO Abbreviation: JPEN J Parenter Enteral Nutr Publication Date: 2007 Sep-Oct |
Date Detail:
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Created Date: 2007-08-22 Completed Date: 2007-12-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7804134 Medline TA: JPEN J Parenter Enteral Nutr Country: United States |
Other Details:
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Languages: eng Pagination: 351-6; discussion 356-7 Citation Subset: IM |
Affiliation:
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Department od Surgery, University of Wisconsin-Madison School of Medicine and Public Health, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Analysis of Variance Animals Blotting, Western Enteral Nutrition* Enzyme-Linked Immunosorbent Assay Gastrostomy Immunoglobulin A, Secretory / biosynthesis*, immunology Interleukin-4 / biosynthesis*, immunology Intestine, Small / immunology* Male Mice Mice, Inbred ICR Parenteral Nutrition* / adverse effects Random Allocation Receptors, Polymeric Immunoglobulin / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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R01 GM53439/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Immunoglobulin A, Secretory; 0/Receptors, Polymeric Immunoglobulin; 207137-56-2/Interleukin-4 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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