| Intestinal permeability and bacterial translocation are uncoupled after small bowel resection. | |
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MedLine Citation:
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PMID: 11877654 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND/PURPOSE: Gut barrier failure and bacterial translocation have been proposed to cause infection and sepsis in patients with the short bowel syndrome. This study tested the hypothesis that permeability is increased in the adapting remnant ileum after massive small bowel resection (SBR). METHODS: Male ICR mice underwent a 50% proximal SBR or sham operation. At 3, 7, and 14 days, the ileum was mounted in an Ussing chamber. Mucosal-to-serosal flux of low (dextran) and high (horseradish peroxidase; HRP) molecular weight markers was determined. Additionally, bacterial translocation was measured by culturing blood, mesenteric lymph nodes, liver, and spleen at 3 and 14 days after SBR or sham operation. RESULTS: Permeability to dextran was reduced immediately after SBR but was no different at later time-points. HRP permeability was no different at any time-point. Translocation of Gram-negative bacteria to the mesenteric lymph nodes and liver was more frequent in the SBR animals 3 and 14 days postoperatively. CONCLUSIONS: Intestinal permeability to macromolecules is not increased after massive SBR, but the rate of translocation to the mesenteric lymph nodes and liver is elevated. This suggests that the mechanism for bacterial translocation after SBR does not involve alterations in gut permeability. |
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Authors:
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David P O'Brien; Lindsey A Nelson; Christopher J Kemp; Jodi L Williams; Quan Wang; Christopher R Erwin; Per-Olof Hasselgren; Brad W Warner |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of pediatric surgery Volume: 37 ISSN: 1531-5037 ISO Abbreviation: J. Pediatr. Surg. Publication Date: 2002 Mar |
Date Detail:
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Created Date: 2002-03-05 Completed Date: 2002-04-09 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0052631 Medline TA: J Pediatr Surg Country: United States |
Other Details:
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Languages: eng Pagination: 390-4 Citation Subset: IM |
Copyright Information:
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Copyright 2002 by W.B. Saunders Company. |
Affiliation:
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Division of Pediatric Surgery, Children's Hospital Medical Center and Shriner's Hospital for Children, Department of Surgery, University of Cincinnati College of Medicine, Cincinnati, OH, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adaptation, Biological
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physiology* Animals Bacterial Translocation / physiology* Dextrans / pharmacokinetics Disease Models, Animal Fluorescein-5-isothiocyanate / analogs & derivatives, pharmacokinetics Horseradish Peroxidase / pharmacokinetics Intestine, Small / metabolism, microbiology*, surgery* Liver / microbiology Lymph Nodes / microbiology Male Mice Mice, Inbred ICR Permeability Postoperative Period Short Bowel Syndrome / metabolism, microbiology*, surgery* Spleen / microbiology |
| Grant Support | |
ID/Acronym/Agency:
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F32 DK09882/DK/NIDDK NIH HHS; R01 DK53234/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/fluorescein isothiocyanate dextran; 3326-32-7/Fluorescein-5-isothiocyanate; 9004-54-0/Dextrans; EC 1.11.1.-/Horseradish Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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