| Intestinal perfusion of beta-carotene in the ferret raises retinoic acid level in portal blood. | |
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MedLine Citation:
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PMID: 8466944 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To determine whether beta-carotene (beta-C) can serve as a source of intestinally-derived retinoic acid (RA), either 15,15'-[14C]beta-C or unlabeled beta-C was perfused through 30 cm jejunal segments of ferrets in vivo. Portal vein blood was sampled periodically via an indwelling catheter. RA was identified in portal blood by comparing retention times in HPLC, by UV absorption, and by derivatization (methylation) and subsequent GC-MS analysis. The RA concentration in the portal blood increased 3-fold with perfusion of beta-C (P < 0.05), and remained at 18 nmol/L during the perfusion of beta-C. The single peak of RA in HPLC was shown to consist of four separate peaks by GC-MS, which may be cis-trans isomers of RA. The concentration of RA in portal blood returned to the initial level (5 nmol/L) after a 2 h period of intestinal perfusion with 5% dextrose. Retinyl ester concentration in portal blood did not change before or after the perfusion, whereas retinol decreased significantly during the perfusion of beta-C. This study clearly indicates that a considerable quantity and number of polar metabolites, including RA, are formed from beta-C in the ferret intestine which are transported via the portal vein to the liver. |
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Authors:
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X D Wang; R M Russell; R P Marini; G Tang; G G Dolnikowski; J G Fox; N I Krinsky |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochimica et biophysica acta Volume: 1167 ISSN: 0006-3002 ISO Abbreviation: Biochim. Biophys. Acta Publication Date: 1993 Apr |
Date Detail:
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Created Date: 1993-05-13 Completed Date: 1993-05-13 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0217513 Medline TA: Biochim Biophys Acta Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 159-64 Citation Subset: IM |
Affiliation:
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USDA Human Nutrition Research Center, School of Nutrition, Tufts University, Boston, MA 02111. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carotenoids / administration & dosage, pharmacology* Ferrets Intestines / drug effects*, metabolism Models, Biological Portal Vein Tretinoin / analogs & derivatives, blood* beta Carotene |
| Grant Support | |
ID/Acronym/Agency:
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R01CA49195-01/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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302-79-4/Tretinoin; 339-16-2/methyl retinoate; 36-88-4/Carotenoids; 7235-40-7/beta Carotene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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