| Intestinal mucus protects Campylobacter jejuni in the ceca of colonized broiler chickens against the bactericidal effects of medium-chain fatty acids. | |
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MedLine Citation:
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PMID: 20460660 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Campylobacter jejuni is the most common cause of bacterial-mediated diarrheal disease worldwide. Because poultry and poultry products are a major source of C. jejuni infections in humans, efforts should be taken to develop strategies to decrease Campylobacter shedding during primary production. For this purpose, the efficacy of medium-chain fatty acids (MCFA) as feed additives to control C. jejuni colonization in broiler chickens was analyzed. First, the antimicrobial activity of the MCFA caproic, caprylic, and capric acid on C. jejuni was evaluated in vitro. Minimal inhibitory concentrations were 0.25 mM for caproic and 0.5 mM for caprylic and capric acids at pH 6.0 and 4 mM for all 3 compounds at pH 7.5. Time-kill curves revealed strong bactericidal properties of the tested compounds toward C. jejuni at pH 6.0. Concentrations as low as 4 mM caprylic and capric acids and 16 mM caproic acid killed all bacteria within 24 h. Capric acid had the highest activity, with concentrations of 4 mM killing all bacteria within the hour. Together these data show a profound bactericidal, dose-dependent activity of the tested MCFA toward C. jejuni in vitro. For this reason, the effect of these 3 MCFA on C. jejuni was evaluated in vivo. The addition of any of the acids to the feed, from 3 d before euthanization, was not capable of reducing cecal Campylobacter colonization in 27-d-old broilers experimentally infected with C. jejuni at 15 d of age. Using a cecal loop model, sodium caprate was not able to reduce cecal Campylobacter counts. When time-kill curves were conducted in the presence of chick intestinal mucus, capric acid was less active against C. jejuni. At 4 mM, all bacteria were killed only after 24 h. Thus, despite the marked bactericidal effect of MCFA in vitro, supplementing these acids to the feed does not reduce cecal Campylobacter colonization in broiler chickens under the applied test conditions, probably due to the protective effect of the mucus layer. |
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Authors:
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D Hermans; A Martel; K Van Deun; M Verlinden; F Van Immerseel; A Garmyn; W Messens; M Heyndrickx; F Haesebrouck; F Pasmans |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Poultry science Volume: 89 ISSN: 0032-5791 ISO Abbreviation: Poult. Sci. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-05-12 Completed Date: 2010-07-08 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0401150 Medline TA: Poult Sci Country: United States |
Other Details:
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Languages: eng Pagination: 1144-55 Citation Subset: IM |
Affiliation:
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Department of Pathology, Bacteriology and Avian Diseases, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820 Merelbeke, Belgium. david.hermans@ugent.be |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animal Feed Animals Anti-Bacterial Agents / chemistry, pharmacology* Campylobacter Infections / microbiology, prevention & control, veterinary* Campylobacter jejuni / drug effects* Cecum / microbiology, physiology* Chickens Dose-Response Relationship, Drug Fatty Acids / chemistry, pharmacology* Female Male Microbial Sensitivity Tests Mucus / physiology* Poultry Diseases / microbiology, prevention & control |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Fatty Acids |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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