| Intestinal mitochondrial apoptotic signaling is activated during oxidative stress. | |
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MedLine Citation:
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PMID: 21400030 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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PURPOSE: Reactive oxygen species (ROS) are thought to contribute to the pathogenesis of necrotizing enterocolitis (NEC). Mitochondria as a major source of intracellular ROS and apoptotic signaling during oxidative stress in NEC have not been investigated. We sought to determine: (1) the effects of oxidative stress on intestinal mitochondrial apoptotic signaling, and (2) the role of growth factors in this process. METHODS: We used Swiss-Webster mice pups, and rat intestinal epithelial (RIE)-1, mitochondrial DNA-depleted RIE-1 cell line (RIE-1-ρ°) and human fetal intestinal epithelial cells (FHs74 Int) for our studies. RESULTS: H(2)O(2) induced apoptosis and ROS production. ROS-mediated activation of apoptotic signaling was significantly attenuated with mitochondrial silencing in RIE-1-ρ° cells. Growth factors, especially IGF-1, attenuated this response to H(2)O(2) in intestinal epithelial cells. CONCLUSIONS: Our findings suggest that mitochondria are a major source of intestinal apoptotic signaling during oxidative stress, and modulating mitochondrial apoptotic responses may help ameliorate the effects of NEC. |
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Authors:
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Naira Baregamian; Jun Song; John Papaconstantinou; Hal K Hawkins; B Mark Evers; Dai H Chung |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-3-13 |
Journal Detail:
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Title: Pediatric surgery international Volume: - ISSN: 1437-9813 ISO Abbreviation: - Publication Date: 2011 Mar |
Date Detail:
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Created Date: 2011-3-14 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8609169 Medline TA: Pediatr Surg Int Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Department of Surgery, The University of Texas Medical Branch, Galveston, TX, 77555, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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