| Intestinal alkaline phosphatase: selective endocytosis from the enterocyte brush border during fat absorption. | |
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MedLine Citation:
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PMID: 17947448 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Absorption of dietary fat in the small intestine is accompanied by a rise of intestinal alkaline phosphatase (IAP) in the serum and of secretion of IAP-containing surfactant-like particles from the enterocytes. In the present work, fat absorption was studied in organ cultured mouse intestinal explants. By immunofluorescence microscopy, fat absorption caused a translocation of IAP from the enterocyte brush border to the interior of the cell, whereas other brush-border enzymes were unaffected. By electron microscopy, the translocation occurred by a rapid (5 min) induction of endocytosis via clathrin-coated pits. By 60 min, IAP was seen in subapical endosomes and along membranes surrounding fat droplets. IAP is a well-known lipid raft-associated protein, and fat absorption was accompanied by a marked change in the density and morphology of the detergent-resistant membranes harboring IAP. A lipid analysis revealed that fat absorption caused a marked increase in the microvillar membrane contents of free fatty acids. In conclusion, fat absorption rapidly induces a transient clathrin-dependent endocytosis via coated pits from the enterocyte brush border. The process selectively internalizes IAP and may contribute to the appearance of the enzyme in serum and surfactant-like particles. |
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Authors:
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Gert H Hansen; Lise-Lotte Niels-Christiansen; Lissi Immerdal; Birthe T Nystrøm; E Michael Danielsen |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-10-18 |
Journal Detail:
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Title: American journal of physiology. Gastrointestinal and liver physiology Volume: 293 ISSN: 0193-1857 ISO Abbreviation: Am. J. Physiol. Gastrointest. Liver Physiol. Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-12-10 Completed Date: 2008-02-06 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901227 Medline TA: Am J Physiol Gastrointest Liver Physiol Country: United States |
Other Details:
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Languages: eng Pagination: G1325-32 Citation Subset: IM |
Affiliation:
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Dept. of Cellular and Molecular Medicine, The Panum Institute University of Copenhagen, Bldg. 6.4, Blegdamsvej 3, DK-2200 Copenhagen N, Denmark. midan@imbg.ku.dk |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Alkaline Phosphatase
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metabolism* Animals Cells, Cultured Dietary Fats / pharmacokinetics* Endocytosis / physiology* Enterocytes / metabolism* Intestine, Small / physiology*, ultrastructure* Mice Microvilli / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Dietary Fats; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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