Document Detail


Intestinal alkaline phosphatase in the colonic mucosa of children with inflammatory bowel disease.
MedLine Citation:
PMID:  22783049     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: To investigate intestinal alkaline phosphatase (iAP) in the intestinal mucosa of children with inflammatory bowel disease (IBD).
METHODS: Colonic biopsy samples were taken from 15 newly diagnosed IBD patients and from 10 healthy controls. In IBD patients, specimens were obtained both from inflamed and non-inflamed areas. The iAP mRNA and protein expression was determined by reverse transcription-polymerase chain reaction and Western blotting analysis, respectively. Tissue localization of iAP and Toll-like receptor (TLR) 4 was investigated by immunofluorescent staining.
RESULTS: The iAP protein level in the inflamed mucosa of children with Crohn's disease (CD) and ulcerative colitis (UC) was significantly decreased when compared with controls (both P < 0.05). Similarly, we found a significantly decreased level of iAP protein in the inflamed mucosa in CD compared with non-inflamed mucosa in CD (P < 0.05). In addition, the iAP protein level in inflamed colonic mucosa in patients with UC was decreased compared with non-inflamed mucosa in patients with CD (P < 0.05). iAP protein levels in the non-inflamed mucosa of patients with CD were similar to controls. iAP mRNA expression in inflamed colonic mucosa of children with CD and UC was not significantly different from that in non-inflamed colonic mucosa with CD. Expression of iAP mRNA in patients with non-inflamed mucosa and in controls were similar. Co-localization of iAP with TLR4 showed intense staining with a dotted-like pattern. iAP was present in the inflamed and non-inflamed mucosa of patients with CD, UC, and in control biopsy specimens, irrespective of whether it was present in the terminal ileum or in the colon. However, the fluorescent signal of TLR4 was more pronounced in the colon compared with the terminal ileum in all groups studied.
CONCLUSION: Lower than normal iAP protein levels in inflamed mucosa of IBD patients may indicate a role for iAP in inflammatory lesions in IBD. Based on our results, administration of exogenous iAP enzyme to patients with the active form of IBD may be a therapeutic option.
Authors:
Kriszta Molnár; Adám Vannay; Beáta Szebeni; Nóra Fanni Bánki; Erna Sziksz; Aron Cseh; Hajnalka Győrffy; Péter László Lakatos; Mária Papp; András Arató; Gábor Veres
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  World journal of gastroenterology : WJG     Volume:  18     ISSN:  2219-2840     ISO Abbreviation:  World J. Gastroenterol.     Publication Date:  2012 Jul 
Date Detail:
Created Date:  2012-07-11     Completed Date:  2012-11-21     Revised Date:  2014-05-20    
Medline Journal Info:
Nlm Unique ID:  100883448     Medline TA:  World J Gastroenterol     Country:  China    
Other Details:
Languages:  eng     Pagination:  3254-9     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Adolescent
Age Factors
Alkaline Phosphatase / analysis*,  genetics
Biopsy
Blotting, Western
Case-Control Studies
Child
Child, Preschool
Colitis / enzymology*,  genetics
Colon / enzymology*
Crohn Disease / enzymology*,  genetics
Down-Regulation
Female
Fluorescent Antibody Technique
GPI-Linked Proteins / analysis,  genetics
Humans
Hungary
Infant
Intestinal Mucosa / enzymology*
Male
RNA, Messenger / analysis
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
Toll-Like Receptor 4 / analysis
Chemical
Reg. No./Substance:
0/GPI-Linked Proteins; 0/RNA, Messenger; 0/TLR4 protein, human; 0/Toll-Like Receptor 4; EC 3.1.3.1/ALPI protein, human; EC 3.1.3.1/Alkaline Phosphatase
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