| Intestinal absorption and biliary secretion of cholesterol in rats with nephrotic syndrome. | |
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MedLine Citation:
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PMID: 9641174 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Nephrotic syndrome (NS) results in hypercholesterolemia which is attributed to increased production and decreased removal of cholesterol-rich lipoproteins. Adjustments in intestinal absorption are reportedly involved in cholesterol homeostasis. We, therefore, studied the intestinal absorption and biliary excretion of cholesterol in NS. METHODS: We studied intestinal absorption (by in vivo perfusion and in vitro everted sac incubation techniques) and biliary secretion (by common bile duct cannulation) of cholesterol in rats with puromycin-induced NS. The results were compared with those obtained from pair-fed control (PF) animals, those given free access to food (NL) or those fed a hypercholerolemic diet (H-chol group). Micellar solutions of Krebs' phosphate buffer containing trace amounts of [14C]inulin and [3H]cholesterol, as well as different concentrations of unlabeled cholesterol, were used for absorption studies. RESULTS: The NS and H-chol groups showed severe and comparable hypercholesterolemia. No significant difference was found in the rate of biliary cholesterol secretion among the study groups. Likewise, the rates of in vivo and in vitro cholesterol absorptions in the NS and H-chol groups were comparable with one another and similar to those found in the NL and PF groups. The rate of in vitro cholesterol absorption was directly proportional to its concentration in the incubation media at low concentrations. However, the absorption rate showed a pattern consistent with saturable transport at high cholesterol concentrations in all groups. CONCLUSIONS: We conclude that intestinal absorption and biliary secretion of cholesterol are not appreciably influenced by either nephrotic or diet-induced hypercholesterolemia in rats. The data further suggest that cholesterol absorption may be a saturable process. |
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Authors:
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M V Pahl; F Oveisi; G Khamiseh; N D Vaziri |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Nephrology, dialysis, transplantation : official publication of the European Dialysis and Transplant Association - European Renal Association Volume: 13 ISSN: 0931-0509 ISO Abbreviation: Nephrol. Dial. Transplant. Publication Date: 1998 Jun |
Date Detail:
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Created Date: 1998-09-09 Completed Date: 1998-09-09 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8706402 Medline TA: Nephrol Dial Transplant Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 1446-51 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of California, Irvine, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biliary Tract / secretion* Cholesterol, Dietary / administration & dosage, pharmacokinetics* Disease Models, Animal Hypercholesterolemia / etiology, physiopathology Intestinal Absorption* Male Nephrotic Syndrome / chemically induced, complications, physiopathology* Perfusion Puromycin / toxicity Rats Rats, Sprague-Dawley |
| Chemical | |
Reg. No./Substance:
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0/Cholesterol, Dietary; 53-79-2/Puromycin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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