| Intestinal absorption of 14C from 14C-phenanthrene, 14C-benzo[a]pyrene and 14C-tetrachlorodibenzo-para-dioxin: approaches with the Caco-2 cell line and with portal absorption measurements in growing pigs. | |
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MedLine Citation:
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PMID: 12956314 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this work was to study the transfer through the intestinal barrier of two polycyclic aromatic hydrocarbons (PAHs) (benzo[a]pyrene and phenanthrene) and a dioxin (2,3,7,8-tetrachlorodibenzo-para-dioxin) which differed in their physicochemical properties. Both in vitro and in vivo assays were performed. For the in vitro study, Caco-2 cells, cultivated on permeable filters, permitted to measure the transepithelial permeability of the studied 14C-labelled molecules. For the in vivo study, portal absorption kinetics were evaluated in pigs fed contamined milk. The results showed that all the molecules were absorbed and demonstrated a differential intestinal absorption for the studied molecules. Phenanthrene appeared to be the fastest and most uptaken compound, followed by benzo[a]pyrene and finally 2,3,7,8-tetrachlorodibenzo-para-dioxin. Their absorption levels were respectively 9.5, 5.2 and 1.4% after a 6 h-exposure in vitro and 86.1, 30.5 and 8.3% in vivo for the 24 h following ingestion. These findings suggest that the physicochemical properties of the xenobiotics and intestinal epithelium play key roles in the selective permeability and in the bioavailability of the tested micropollutants. |
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Authors:
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Séverine Cavret; Claire Laurent; Cyril Feidt; François Laurent; Guido Rychen |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Reproduction, nutrition, development Volume: 43 ISSN: 0926-5287 ISO Abbreviation: Reprod. Nutr. Dev. Publication Date: 2003 Mar-Apr |
Date Detail:
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Created Date: 2003-09-05 Completed Date: 2003-12-02 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 8913069 Medline TA: Reprod Nutr Dev Country: France |
Other Details:
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Languages: eng Pagination: 145-54 Citation Subset: IM |
Affiliation:
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Laboratoire de Sciences Animales, INPL-UHP-INRA, Ecole Nationale Supérieure d'Agronomie et des Industries Alimentaires, BP 172, 54505 Vandoeuvre-lès-Nancy, France. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Absorption Animals Arteries Benzo(a)pyrene / analysis, chemistry, pharmacokinetics* Caco-2 Cells Carbon Radioisotopes / pharmacokinetics* Chemistry, Physical Epithelium Humans Intestinal Absorption* Models, Biological Permeability Phenanthrenes / blood, chemistry, pharmacokinetics* Physicochemical Phenomena Portal Vein* Swine Tetrachlorodibenzodioxin / blood, chemistry, pharmacokinetics* |
| Chemical | |
Reg. No./Substance:
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0/Carbon Radioisotopes; 0/Phenanthrenes; 1746-01-6/Tetrachlorodibenzodioxin; 50-32-8/Benzo(a)pyrene; 85-01-8/phenanthrene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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