Document Detail

Intestinal Neurogenin 3 directs differentiation of a bipotential secretory progenitor to endocrine cell rather than goblet cell fate.
MedLine Citation:
PMID:  17706959     Owner:  NLM     Status:  MEDLINE    
Neurogenin 3 is essential for enteroendocrine cell development; however, it is unknown whether this transcription factor is sufficient to induce an endocrine program in the intestine or how it affects the development of other epithelial cells originating from common progenitors. In this study, the mouse villin promoter was used to drive Neurogenin 3 expression throughout the developing epithelium to measure the affect on cell fate. Although the general morphology of the intestine was unchanged, transgenic founder embryos displayed increased numbers of cells expressing the pan-endocrine marker chromogranin A. Accordingly, expression of several hormones and pro-endocrine transcription factors was increased in the transgenics suggesting that Neurogenin 3 stimulated a program of terminal enteroendocrine cell development. To test whether increased endocrine cell differentiation affected the development of other secretory cell lineages, we quantified goblet cells, the only other secretory cell formed in embryonic intestine. The Neurogenin 3-expressing transgenics had decreased numbers of goblet cells in correspondence to the increase in endocrine cells, with no change in the total secretory cell numbers. Thus, our data suggest that Neurogenin 3 can redirect the differentiation of bipotential secretory progenitors to endocrine rather than goblet cell fate.
Lymari López-Díaz; Renu N Jain; Theresa M Keeley; Kelli L VanDussen; Cynthia S Brunkan; Deborah L Gumucio; Linda C Samuelson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2007-07-24
Journal Detail:
Title:  Developmental biology     Volume:  309     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2007 Sep 
Date Detail:
Created Date:  2007-09-10     Completed Date:  2008-01-08     Revised Date:  2014-09-14    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  298-305     Citation Subset:  IM    
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MeSH Terms
Basic Helix-Loop-Helix Transcription Factors / genetics,  physiology*
Cell Differentiation
Enteroendocrine Cells / cytology*,  metabolism
Goblet Cells / cytology*,  metabolism
Homeodomain Proteins / metabolism
Intestines / cytology*,  embryology,  metabolism
Mice, Transgenic
Nerve Tissue Proteins / genetics,  physiology*
Paired Box Transcription Factors / metabolism
Stem Cells / cytology*,  metabolism
Trans-Activators / metabolism
Grant Support
P01 DK062041/DK/NIDDK NIH HHS; P01 DK062041-010002/DK/NIDDK NIH HHS; P01-DK06241/DK/NIDDK NIH HHS; R01 DK056882/DK/NIDDK NIH HHS; R01 DK056882-05A2/DK/NIDDK NIH HHS; R01 DK056882-06/DK/NIDDK NIH HHS; R01 DK056882-07/DK/NIDDK NIH HHS; R01 DK078927/DK/NIDDK NIH HHS; R01 DK078927-01A1/DK/NIDDK NIH HHS; R01 DK078927-02/DK/NIDDK NIH HHS; T32-GM008322/GM/NIGMS NIH HHS; T32-GM07315/GM/NIGMS NIH HHS
Reg. No./Substance:
0/Basic Helix-Loop-Helix Transcription Factors; 0/Homeodomain Proteins; 0/Nerve Tissue Proteins; 0/Neurog3 protein, mouse; 0/Paired Box Transcription Factors; 0/Pax4 protein, mouse; 0/Trans-Activators; 0/pancreatic and duodenal homeobox 1 protein

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