Document Detail

Intervention of pyrrolidine dithiocarbamate and tetrandrine on cellular calcium overload of pancreatic acinar cells induced by serum and ascitic fluid from rats with acute pancreatitis.
MedLine Citation:
PMID:  19196399     Owner:  NLM     Status:  MEDLINE    
BACKGROUND AND AIM: To investigate the effects of serum and ascitic fluid from rats with acute pancreatitis (AP) on cellular free calcium concentration ([Ca(2+)]i) of isolated rat pancreatic acinar cells, and the intervention of pyrrolidine dithiocarbamate (PDTC) and tetrandrine (Tet) to cellular calcium overload in AP. METHODS: AP was induced in Sprague-Dawley rats with a retrograde pancreatic duct injection of 3% sodium deoxycholate, and confirmed by histopathological examination and amylase activity assay. The rat serum and ascitic fluid were collected at 1, 5 and 10 h after AP induction, and used as irritants on isolated rat pancreatic acinar cells. The effects on intracellular [Ca(2+)]i, and cell viability were examined. Then, the antagonistic effects of different concentrations of PDTC and Tet were assessed. RESULTS: The irritation with AP serum and ascitic fluid reduced the survival rate of the isolated rat pancreatic acinar cells and increased the cellular [Ca(2+)]i significantly (P < 0.05). As AP induction course prolonged, the stimulation effect of the AP serum and ascitic fluid intensified. In the pretreated acinar cells with PDTC or Tet, the decreased cell vitality reverted. The elevation of [Ca(2+)]i in the acinar cells significantly ameliorated (significant, P < 0.05; very significant, P < 0.01). CONCLUSION: The serum and ascitic fluid from AP rats drastically elevate the [Ca(2+)]i in isolated pancreatic acinar cells and decrease cell vitality, while the pretreatment of cells with PDTC and Tet offsets the calcium overload irritated by the AP serum and ascitic fluid and protects these isolated acinar cells.
Yong-Yu Li; Xin-Yuan Lu; Xue-Jin Li; Yan-Na Li; Kun Li; Chang-Jie Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of gastroenterology and hepatology     Volume:  24     ISSN:  1440-1746     ISO Abbreviation:  J. Gastroenterol. Hepatol.     Publication Date:  2009 Jan 
Date Detail:
Created Date:  2009-02-06     Completed Date:  2009-05-07     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8607909     Medline TA:  J Gastroenterol Hepatol     Country:  Australia    
Other Details:
Languages:  eng     Pagination:  155-65     Citation Subset:  IM    
Department of Pathophysiology, Institute of Digestive Disease, Medical School of Tongji University, Shanghai, China.
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MeSH Terms
Acute Disease
Amylases / blood
Anti-Inflammatory Agents / pharmacology*
Ascitic Fluid / metabolism*
Benzylisoquinolines / pharmacology*
Calcium / metabolism*
Calcium Channel Blockers / pharmacology*
Cell Survival / drug effects
Cells, Cultured
Deoxycholic Acid
Disease Models, Animal
NF-kappa B / antagonists & inhibitors
Pancreas, Exocrine / drug effects*,  metabolism,  pathology
Pancreatitis / blood,  chemically induced,  metabolism*,  pathology
Pyrrolidines / pharmacology*
Rats, Sprague-Dawley
Thiocarbamates / pharmacology*
Time Factors
Reg. No./Substance:
0/Anti-Inflammatory Agents; 0/Benzylisoquinolines; 0/Calcium Channel Blockers; 0/NF-kappa B; 0/Pyrrolidines; 0/Thiocarbamates; 25769-03-3/pyrrolidine dithiocarbamic acid; 518-34-3/tetrandrine; 7440-70-2/Calcium; 83-44-3/Deoxycholic Acid; EC 3.2.1.-/Amylases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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