| Intervention of oxygen-control ability to radiation sensitivity, cell aging and cell transformation. | |
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MedLine Citation:
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PMID: 19194067 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Oxygen is essential for life, and cells have therefore developed numerous adaptive responses to oxygen change. Here, we examined the difference in oxygen-control functions of human (HE), mouse (ME), and Syrian hamster embryo (SHE) cells cultured under different oxygen conditions (0.5%, 2% and 20%), and also examined whether oxygen tensions contributed to cellular lifespan and transformation. HE cells had their replicative lifespan slightly extended under hypoxic (0.5% and 2% oxygen) conditions, but were not immortalized under any of the oxygen concentrations. On the other hand, although ME cells cultured under 20% oxygen tension decreased their proliferation potency temporarily at early stage, all rodent cells were immortalized and acquired anchorage-independency, regardless of oxygen tension. These results suggest that cellular oxygen control function is related to sensitivities cellular immortalization and transformation. To understand intervention of oxygen control ability on cellular immortalization and transformation, we examined the intracellular oxidative level, mitochondria functions and radiation sensitivity. Intracellular oxidative levels of hypoxically cultured rodent cells were significantly enhanced. Mitochondrial membrane potential was altered depend on oxygen tensions, but the change was not parallel to mitochondria number in rodent cells. ME cells were particularly sensitive to oxygen change, and showed a clear oxygen effect on the X-ray survival. However, there was no difference in frequency of radiation-induced micronuclei between HE and ME cells. These results suggest that the response to oxygen change differs markedly in HE and rodent cells. |
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Authors:
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Hanako Yoshii; Masami Watanabe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-04 |
Journal Detail:
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Title: Journal of radiation research Volume: 50 ISSN: 0449-3060 ISO Abbreviation: J. Radiat. Res. Publication Date: 2009 Mar |
Date Detail:
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Created Date: 2009-04-06 Completed Date: 2009-07-01 Revised Date: 2012-03-28 |
Medline Journal Info:
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Nlm Unique ID: 0376611 Medline TA: J Radiat Res Country: Japan |
Other Details:
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Languages: eng Pagination: 127-37 Citation Subset: IM |
Affiliation:
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Research Reactor Institute, Kyoto University, Kumatori-cho, Sennan-gun, Osaka, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anoxia Cell Aging* Cell Survival Cell Transformation, Neoplastic* Cricetinae Humans Membrane Potentials Mesocricetus Mice Mice, Inbred C57BL Micronucleus Tests Mitochondria / metabolism Models, Biological Oxygen / metabolism* X-Rays |
| Chemical | |
Reg. No./Substance:
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7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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