Document Detail

Intervention of oxygen-control ability to radiation sensitivity, cell aging and cell transformation.
MedLine Citation:
PMID:  19194067     Owner:  NLM     Status:  MEDLINE    
Oxygen is essential for life, and cells have therefore developed numerous adaptive responses to oxygen change. Here, we examined the difference in oxygen-control functions of human (HE), mouse (ME), and Syrian hamster embryo (SHE) cells cultured under different oxygen conditions (0.5%, 2% and 20%), and also examined whether oxygen tensions contributed to cellular lifespan and transformation. HE cells had their replicative lifespan slightly extended under hypoxic (0.5% and 2% oxygen) conditions, but were not immortalized under any of the oxygen concentrations. On the other hand, although ME cells cultured under 20% oxygen tension decreased their proliferation potency temporarily at early stage, all rodent cells were immortalized and acquired anchorage-independency, regardless of oxygen tension. These results suggest that cellular oxygen control function is related to sensitivities cellular immortalization and transformation. To understand intervention of oxygen control ability on cellular immortalization and transformation, we examined the intracellular oxidative level, mitochondria functions and radiation sensitivity. Intracellular oxidative levels of hypoxically cultured rodent cells were significantly enhanced. Mitochondrial membrane potential was altered depend on oxygen tensions, but the change was not parallel to mitochondria number in rodent cells. ME cells were particularly sensitive to oxygen change, and showed a clear oxygen effect on the X-ray survival. However, there was no difference in frequency of radiation-induced micronuclei between HE and ME cells. These results suggest that the response to oxygen change differs markedly in HE and rodent cells.
Hanako Yoshii; Masami Watanabe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-02-04
Journal Detail:
Title:  Journal of radiation research     Volume:  50     ISSN:  0449-3060     ISO Abbreviation:  J. Radiat. Res.     Publication Date:  2009 Mar 
Date Detail:
Created Date:  2009-04-06     Completed Date:  2009-07-01     Revised Date:  2012-03-28    
Medline Journal Info:
Nlm Unique ID:  0376611     Medline TA:  J Radiat Res     Country:  Japan    
Other Details:
Languages:  eng     Pagination:  127-37     Citation Subset:  IM    
Research Reactor Institute, Kyoto University, Kumatori-cho, Sennan-gun, Osaka, Japan.
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MeSH Terms
Cell Aging*
Cell Survival
Cell Transformation, Neoplastic*
Membrane Potentials
Mice, Inbred C57BL
Micronucleus Tests
Mitochondria / metabolism
Models, Biological
Oxygen / metabolism*
Reg. No./Substance:

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