Document Detail


Interstitial colocalization of two cervid satellite DNAs involved in the genesis of the Indian muntjac karyotype.
MedLine Citation:
PMID:  10997777     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A number of repetitive DNA clones were generated from PCR amplifications of Indian muntjac genomic DNA using primer sequences derived from a white tailed deer satellite II DNA sequence. One clone (Mmv-0.7) was characterized and shown to be a cervid satellite II DNA clone. Multiple colored FISH studies with cervid satellite I (C5) and this satellite II clone (Mmv-0.7) to Chinese muntjac metaphase chromosomes localized both satellite DNAs at the pericentromeric regions of all chromosomes except for chromosome 3 and the Y chromosome, whereas chromosome 3 exhibited pericentromeric satellite II DNA only. Where distinguishable, the pericentromeric satellite II signals appeared terminally oriented with respect to satellite I. Six pairs of Chinese muntjac autosomes had interstitial satellite I sites with four of these autosomal pairs (chromosomes 1, 2 and two other smaller autosomal pairs) also exhibiting interstitial satellite II signals. An interstitial site on the X chromosome was found to have satellite II signals. For the Indian muntjac chromosomes, FISH studies revealed a pericentromeric hybridization for satellites I and II as well as 27 distinct interstitial hybridization sites, each having at least one of the satellite DNAs. These data were used to more precisely define the chromosome fusion-associated breakpoints that presumably led to the formation of the present-day Indian muntjac karyotype. It further hints at the possibility that the Indian muntjac karyotype may have evolved directly from a 2n = 70 ancestral karyotype rather than from an intermediate 2n = 46 Chinese muntjac-like karyotype.
Authors:
Y C Li; C Lee; D Sanoudou; T H Hseu; S Y Li; C C Lin; T H Hsu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology     Volume:  8     ISSN:  0967-3849     ISO Abbreviation:  Chromosome Res.     Publication Date:  2000  
Date Detail:
Created Date:  2000-12-18     Completed Date:  2001-01-11     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9313452     Medline TA:  Chromosome Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  363-73     Citation Subset:  IM    
Affiliation:
Department of Life Sciences, Chung Shan Medical and Dental College, Taichung, Taiwan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Blotting, Southern
Centromere
Chromosomes / ultrastructure
DNA Primers
DNA, Satellite*
Deer
Female
In Situ Hybridization, Fluorescence
Karyotyping
Male
Models, Genetic
Molecular Sequence Data
Muntjacs
Polymerase Chain Reaction
Repetitive Sequences, Nucleic Acid
Sequence Homology, Nucleic Acid
Chemical
Reg. No./Substance:
0/DNA Primers; 0/DNA, Satellite
Comments/Corrections
Erratum In:
Chromosome Res 2001;9(1):83
Note: Hsu TH [corrected to Hseu TH]

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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