| Interstitial adenosine in guinea pig hearts: an index obtained by epicardial disks. | |
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MedLine Citation:
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PMID: 2240246 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Epicardial porous disks were used to estimate left ventricular interstitial fluid adenosine in the isolated guinea pig heart perfused at constant flow. To validate this technique, adenosine was infused with 0.5 microM dipyridamole and 5 microM erythro-4-(2-hydroxy-3-nonyl)adenine hydrochloride (EHNA) to inhibit adenosine uptake and deamination, respectively. Under these conditions, with 6 or 12 microM adenosine in the arterial infusate, the venous and steady-state left ventricular disk adenosine values equaled those of the perfusion fluid, reflecting the expected equilibration between vascular and left ventricular interstitial fluid (disk) compartments. The concentration of endogenous adenosine in the interstitial fluid as estimated by the epicardial disks under control conditions was 0.28 +/- 0.03 microM with a concomitant coronary sinus concentration of 0.004 +/- 0.001 microM. We conclude that a marked adenosine gradient exists between the interstitial fluid and the vascular space and that disk adenosine measurements provide a useful index of left ventricular interstitial fluid adenosine. |
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Authors:
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C S Tietjan; C G Tribble; J M Gidday; C L Phillips; L Belardinelli; R Rubio; R M Berne |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: The American journal of physiology Volume: 259 ISSN: 0002-9513 ISO Abbreviation: Am. J. Physiol. Publication Date: 1990 Nov |
Date Detail:
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Created Date: 1990-12-13 Completed Date: 1990-12-13 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0370511 Medline TA: Am J Physiol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: H1471-6 Citation Subset: IM |
Affiliation:
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Department of Physiology, University of Virginia, Charlottesville 22908. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine
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metabolism* Animals Arabinonucleosides / metabolism Dipyridamole / pharmacology Extracellular Space / metabolism* Guinea Pigs Homeostasis Myocardium / metabolism* Osmolar Concentration Physiology / instrumentation, methods |
| Chemical | |
Reg. No./Substance:
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0/Arabinonucleosides; 58-32-2/Dipyridamole; 58-61-7/Adenosine; 7013-16-3/hypoxanthine arabinoside |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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