| Interspecies somatic cell nucleus transfer with porcine oocytes as recipients: A novel bioassay system for assessing the competence of canine somatic cells to develop into embryos. | |
| | |
MedLine Citation:
|
PMID: 19524287 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Interspecies somatic cell nucleus transfer (iSCNT) could be a useful bioassay system for assessing the ability of mammalian somatic cells to develop into embryos. To examine this possibility, we performed canine iSCNT using porcine oocytes, allowed to mature in vitro, as recipients. Canine fibroblasts from the tail tips and dewclaws of a female poodle (Fp) and a male poodle (Mp) were used as donors. We demonstrated that the use of porcine oocytes induced blastocyst formation in the iSCNT embryos cultured in porcine zygote medium-3. In Fp and Mp, the rate of blastocyst formation from cleaved embryos (Fp: 6.3% vs. 22.4%; and Mp: 26.1% vs. 52.4%) and the number of cells at the blastocyst stage (Fp: 30.7 vs. 60.0; and Mp: 27.2 vs. 40.1) were higher in the embryos derived from dewclaw cells than in those derived from tail-tip cells (P<0.05). The use of donor cells of any type in later passages decreased the rate of blastocyst formation. Treatment with trichostatin-A did not improve the rate of blastocyst formation from cleaved dewclaw cell-derived embryos but did so in the embryos derived from the tail-tip cells of Fp. Only blastocysts derived from dewclaw cells of Mp developed outgrowths. However, outgrowth formation was retrieved in the embryos derived from dewclaw cells of Fp by aggregation at the 4-cell stage. We inferred that iSCNT performed using porcine oocytes as recipients could represent a novel bioassay system for evaluating the developmental competence of canine somatic cells. |
| | |
Authors:
|
S Sugimura; K Narita; H Yamashiro; A Sugawara; T Shoji; Y Terashita; K Nishimori; T Konno; M Yoshida; E Sato |
Related Documents
:
|
1138447 - Lymphocyte stimulation with skin cells. a function of maturation. 11556527 - Total ablation of paternal accessory sex glands curtails developmental potential in pre... 8045337 - Mesendoderm cell and archenteron formation in isolated blastomeres from the shrimp sicy... 18576827 - Redescription of dicyemennea nouveli (phylum: dicyemida) from enteroctopus dofleini (mo... 17577207 - Differential enhancement of neural and photoreceptor cell differentiation of cultured p... 9765357 - Differential effect of ethanol on pc12 cell death. |
Publication Detail:
|
Type: Evaluation Studies; Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-12 |
Journal Detail:
|
Title: Theriogenology Volume: 72 ISSN: 1879-3231 ISO Abbreviation: Theriogenology Publication Date: 2009 Sep |
Date Detail:
|
Created Date: 2009-08-03 Completed Date: 2009-11-17 Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 0421510 Medline TA: Theriogenology Country: United States |
Other Details:
|
Languages: eng Pagination: 549-59 Citation Subset: IM |
Affiliation:
|
Laboratory of Animal Reproduction, Graduate School of Agricultural Science, Tohoku University, 1-1 Tsutsumidori-amamiyamachi, Aoba-ku, Sendai 981-8555, Japan. s0sugimr@nlbc.go.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Biological Assay / methods* Dogs Embryo Culture Techniques Embryo, Mammalian* / drug effects Embryonic Development Female Fibroblasts Hydroxamic Acids / pharmacology Male Nuclear Transfer Techniques* Oocytes / cytology*, physiology Swine / genetics* |
| Chemical | |
Reg. No./Substance:
|
0/Hydroxamic Acids; 58880-19-6/trichostatin A |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Anti-angiogenesis therapy with bevacizumab for patients with ovarian granulosa cell tumors.
Next Document: The use of three-dimensional nanostructures to instruct cells to produce extracellular matrix for re...