Document Detail

Intersection of RNA processing and the type II fatty acid synthesis pathway in yeast mitochondria.
MedLine Citation:
PMID:  18779316     Owner:  NLM     Status:  MEDLINE    
Distinct metabolic pathways can intersect in ways that allow hierarchical or reciprocal regulation. In a screen of respiration-deficient Saccharomyces cerevisiae gene deletion strains for defects in mitochondrial RNA processing, we found that lack of any enzyme in the mitochondrial fatty acid type II biosynthetic pathway (FAS II) led to inefficient 5' processing of mitochondrial precursor tRNAs by RNase P. In particular, the precursor containing both RNase P RNA (RPM1) and tRNA(Pro) accumulated dramatically. Subsequent Pet127-driven 5' processing of RPM1 was blocked. The FAS II pathway defects resulted in the loss of lipoic acid attachment to subunits of three key mitochondrial enzymes, which suggests that the octanoic acid produced by the pathway is the sole precursor for lipoic acid synthesis and attachment. The protein component of yeast mitochondrial RNase P, Rpm2, is not modified by lipoic acid in the wild-type strain, and it is imported in FAS II mutant strains. Thus, a product of the FAS II pathway is required for RNase P RNA maturation, which positively affects RNase P activity. In addition, a product is required for lipoic acid production, which is needed for the activity of pyruvate dehydrogenase, which feeds acetyl-coenzyme A into the FAS II pathway. These two positive feedback cycles may provide switch-like control of mitochondrial gene expression in response to the metabolic state of the cell.
Melissa S Schonauer; Alexander J Kastaniotis; J Kalervo Hiltunen; Carol L Dieckmann
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-09-08
Journal Detail:
Title:  Molecular and cellular biology     Volume:  28     ISSN:  1098-5549     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-15     Completed Date:  2008-11-07     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6646-57     Citation Subset:  IM    
Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, AZ 85721-0106, USA.
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MeSH Terms
Blotting, Northern
Fatty Acid Synthetase Complex, Type II / genetics,  metabolism*
Gene Deletion
Gene Expression Regulation, Fungal
Green Fluorescent Proteins / metabolism
Hydro-Lyases / metabolism
Mitochondria / enzymology*,  genetics
Mitochondrial Proteins / metabolism
Models, Biological
Protein Transport
RNA Precursors / metabolism
RNA Processing, Post-Transcriptional*
RNA, Fungal / metabolism
RNA, Messenger / genetics,  metabolism
RNA, Transfer / metabolism
Recombinant Fusion Proteins / metabolism
Ribonuclease P / metabolism
Saccharomyces cerevisiae / enzymology*,  genetics
Saccharomyces cerevisiae Proteins / metabolism
Trans-Activators / metabolism
Grant Support
Reg. No./Substance:
0/Mitochondrial Proteins; 0/PET127 protein, S cerevisiae; 0/RNA Precursors; 0/RNA, Fungal; 0/RNA, Messenger; 0/Recombinant Fusion Proteins; 0/Saccharomyces cerevisiae Proteins; 0/Trans-Activators; 147336-22-9/Green Fluorescent Proteins; 9014-25-9/RNA, Transfer; EC protein, S cerevisiae; EC P; EC 4.2.1.-/Htd2 protein, S cerevisiae; EC 4.2.1.-/Hydro-Lyases; EC 6.-/Fatty Acid Synthetase Complex, Type II

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