Document Detail


Intersection of RNA processing and the type II fatty acid synthesis pathway in yeast mitochondria.
MedLine Citation:
PMID:  18779316     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Distinct metabolic pathways can intersect in ways that allow hierarchical or reciprocal regulation. In a screen of respiration-deficient Saccharomyces cerevisiae gene deletion strains for defects in mitochondrial RNA processing, we found that lack of any enzyme in the mitochondrial fatty acid type II biosynthetic pathway (FAS II) led to inefficient 5' processing of mitochondrial precursor tRNAs by RNase P. In particular, the precursor containing both RNase P RNA (RPM1) and tRNA(Pro) accumulated dramatically. Subsequent Pet127-driven 5' processing of RPM1 was blocked. The FAS II pathway defects resulted in the loss of lipoic acid attachment to subunits of three key mitochondrial enzymes, which suggests that the octanoic acid produced by the pathway is the sole precursor for lipoic acid synthesis and attachment. The protein component of yeast mitochondrial RNase P, Rpm2, is not modified by lipoic acid in the wild-type strain, and it is imported in FAS II mutant strains. Thus, a product of the FAS II pathway is required for RNase P RNA maturation, which positively affects RNase P activity. In addition, a product is required for lipoic acid production, which is needed for the activity of pyruvate dehydrogenase, which feeds acetyl-coenzyme A into the FAS II pathway. These two positive feedback cycles may provide switch-like control of mitochondrial gene expression in response to the metabolic state of the cell.
Authors:
Melissa S Schonauer; Alexander J Kastaniotis; J Kalervo Hiltunen; Carol L Dieckmann
Related Documents :
10438506 - The binding of arachidonic acid in the cyclooxygenase active site of mouse prostaglandi...
11095706 - The activation mechanism of rat vanilloid receptor 1 by capsaicin involves the pore dom...
14690446 - Glutamate 346 of human na+-h+ exchanger nhe1 is crucial for modulating both the affinit...
10930666 - Mutations occurring during serial passage of japanese equine infectious anemia virus in...
23531206 - 6:2 and 8:2 fluorotelomer alcohol anaerobic biotransformation in digester sludge from a...
1331036 - Possible physiological roles of aspartase, nad- and nadp-requiring glutamate dehydrogen...
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2008-09-08
Journal Detail:
Title:  Molecular and cellular biology     Volume:  28     ISSN:  1098-5549     ISO Abbreviation:  Mol. Cell. Biol.     Publication Date:  2008 Nov 
Date Detail:
Created Date:  2008-10-15     Completed Date:  2008-11-07     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  8109087     Medline TA:  Mol Cell Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  6646-57     Citation Subset:  IM    
Affiliation:
Department of Biochemistry and Molecular Biophysics, University of Arizona, Tucson, AZ 85721-0106, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Blotting, Northern
Fatty Acid Synthetase Complex, Type II / genetics,  metabolism*
Gene Deletion
Gene Expression Regulation, Fungal
Green Fluorescent Proteins / metabolism
Hydro-Lyases / metabolism
Lipoylation
Mitochondria / enzymology*,  genetics
Mitochondrial Proteins / metabolism
Models, Biological
Protein Transport
RNA Precursors / metabolism
RNA Processing, Post-Transcriptional*
RNA, Fungal / metabolism
RNA, Messenger / genetics,  metabolism
RNA, Transfer / metabolism
Recombinant Fusion Proteins / metabolism
Ribonuclease P / metabolism
Saccharomyces cerevisiae / enzymology*,  genetics
Saccharomyces cerevisiae Proteins / metabolism
Trans-Activators / metabolism
Grant Support
ID/Acronym/Agency:
GM34893/GM/NIGMS NIH HHS; T3208659//PHS HHS
Chemical
Reg. No./Substance:
0/Mitochondrial Proteins; 0/PET127 protein, S cerevisiae; 0/RNA Precursors; 0/RNA, Fungal; 0/RNA, Messenger; 0/Recombinant Fusion Proteins; 0/Saccharomyces cerevisiae Proteins; 0/Trans-Activators; 147336-22-9/Green Fluorescent Proteins; 9014-25-9/RNA, Transfer; EC 3.1.26.5/RPM2 protein, S cerevisiae; EC 3.1.26.5/Ribonuclease P; EC 4.2.1.-/Htd2 protein, S cerevisiae; EC 4.2.1.-/Hydro-Lyases; EC 6.-/Fatty Acid Synthetase Complex, Type II
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The ING4 tumor suppressor attenuates NF-kappaB activity at the promoters of target genes.
Next Document:  p53 regulates Toll-like receptor 3 expression and function in human epithelial cell lines.