| Interruption of NFkappaB-STAT1 signaling mediates EGF-induced cell-cycle arrest. | |
| | |
MedLine Citation:
|
PMID: 10825242 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
It is known that EGF induces the cell-cycle arrest in A431 cells that possess high numbers of EGF receptors and it was previously suggested that p21/WAF1 protein was a major effector molecule of the EGF-mediated cell-cycle arrest of A431 cells. Here, we further investigate this phenomenon using the decoy double-strand oligonucleotides for STAT-binding sequence (STAT decoy) and IkappaB, an inhibitor of the nuclear factor kappa B (NFkappaB). Addition of STAT decoy restored EGF-induced A431 cell-growth arrest. Interestingly, infection of adenovirus vectors to express IkappaB (AxIkappaBalphaDeltaN) as the inhibitor of NFkappaB also reversed the A431 cell-growth inhibition. The individual treatment of two inhibitors partially inhibited the WAF1 gene expression, whereas simultaneous treatment of two inhibitors exhibited more efficient inhibition. These observations suggest the activation of NFkappaB via IkappaB degradation and STAT1 via specific receptor kinase activity synergistically induce WAF1 gene expression in A431 cells. Thus, NFkappaB and STAT1 pathways mutually interact to play an important role in the EGF-induced intracellular reaction. |
| | |
Authors:
|
M Ohtsubo; A Takayanagi; S Gamou; N Shimizu |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Journal of cellular physiology Volume: 184 ISSN: 0021-9541 ISO Abbreviation: J. Cell. Physiol. Publication Date: 2000 Jul |
Date Detail:
|
Created Date: 2000-07-03 Completed Date: 2000-07-03 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 0050222 Medline TA: J Cell Physiol Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 131-7 Citation Subset: IM |
Copyright Information:
|
Copyright 2000 Wiley-Liss, Inc. |
Affiliation:
|
Department of Molecular Biology, Keio University School of Medicine, Tokyo, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Carcinoma, Squamous Cell Cell Cycle / drug effects, physiology* Cell Division Cyclin-Dependent Kinase Inhibitor p21 Cyclins / genetics, metabolism DNA-Binding Proteins / genetics, metabolism*, physiology Enzyme Inhibitors / metabolism Epidermal Growth Factor / pharmacology* Genes, Reporter Humans I-kappa B Proteins* NF-kappa B / metabolism* Phosphorylation STAT1 Transcription Factor Signal Transduction* Trans-Activators / metabolism* Transfection Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
|
0/CDKN1A protein, human; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Cyclins; 0/DNA-Binding Proteins; 0/Enzyme Inhibitors; 0/I-kappa B Proteins; 0/NF-kappa B; 0/STAT1 Transcription Factor; 0/STAT1 protein, human; 0/Trans-Activators; 139874-52-5/NF-kappaB inhibitor alpha; 62229-50-9/Epidermal Growth Factor |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Intracellular osteopontin is an integral component of the CD44-ERM complex involved in cell migratio...
Next Document: 252Cf plasma desorption mass spectrometric study of interactions of steroid glycosides with amino ac...