Document Detail


Interrogation of living cells using alternating current scanning electrochemical microscopy (AC-SECM).
MedLine Citation:
PMID:  18004408     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In this paper we present the application of alternating current scanning electrochemical microscopy (AC-SECM) to the study of living cells. Commercial AFM instrumentation was modified to allow for performing robust AC-SECM measurements. Constant height AC imaging of the Cos-7 cells, performed directly in cell culture medium without the addition of a redox mediator, provided topographical information of the cell. Stationary tip measurements on the AC current were carried out to investigate the cellular activity of a single cell. The dependence of AC current magnitude on tip-to-sample separation distance was used to monitor real time changes in cell height of individual Cos-7 cells. Furthermore, AC-SECM was employed to observe changes in metabolic cellular activity stimulated by ethanol and phorbol-1,2-myristate-acetate-3. The effect of changing cellular activity on constant height AC-SECM imaging was also studied.
Authors:
Piotr M Diakowski; Zhifeng Ding
Related Documents :
15834518 - Micro/nanofluidic device for single-cell-based assay.
17506938 - Real-time detection of alpha1a-ar movement stimulated by phenylephrine in single living...
3392958 - Study of living single cells in culture: automated recognition of cell behavior.
11807938 - Drastic change of local stiffness distribution correlating to cell migration in living ...
7507418 - Altered morphology of vegetative amoebae induced by increased expression of the dictyos...
23018598 - Physical and chemical microenvironmental cues orthogonally control the degree and durat...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-09
Journal Detail:
Title:  Physical chemistry chemical physics : PCCP     Volume:  9     ISSN:  1463-9076     ISO Abbreviation:  Phys Chem Chem Phys     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-16     Completed Date:  2008-02-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100888160     Medline TA:  Phys Chem Chem Phys     Country:  England    
Other Details:
Languages:  eng     Pagination:  5966-74     Citation Subset:  IM    
Affiliation:
Department of Chemistry, The University of Western Ontario, London, ON, Canada.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Biosensing Techniques / methods*
COS Cells / metabolism,  ultrastructure
Cell Physiological Phenomena*
Cercopithecus aethiops
Electrochemistry
Ethanol / pharmacology
Microscopy, Electron, Scanning / methods
Tetradecanoylphorbol Acetate / pharmacology
Chemical
Reg. No./Substance:
16561-29-8/Tetradecanoylphorbol Acetate; 64-17-5/Ethanol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Frequency-dependent response properties and excitation energies from one-electron density matrix fun...
Next Document:  Hydrogen abstraction from deoxyribose by a neighbouring uracil-5-yl radical.