Document Detail

Interrogation of living cells using alternating current scanning electrochemical microscopy (AC-SECM).
MedLine Citation:
PMID:  18004408     Owner:  NLM     Status:  MEDLINE    
In this paper we present the application of alternating current scanning electrochemical microscopy (AC-SECM) to the study of living cells. Commercial AFM instrumentation was modified to allow for performing robust AC-SECM measurements. Constant height AC imaging of the Cos-7 cells, performed directly in cell culture medium without the addition of a redox mediator, provided topographical information of the cell. Stationary tip measurements on the AC current were carried out to investigate the cellular activity of a single cell. The dependence of AC current magnitude on tip-to-sample separation distance was used to monitor real time changes in cell height of individual Cos-7 cells. Furthermore, AC-SECM was employed to observe changes in metabolic cellular activity stimulated by ethanol and phorbol-1,2-myristate-acetate-3. The effect of changing cellular activity on constant height AC-SECM imaging was also studied.
Piotr M Diakowski; Zhifeng Ding
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2007-10-09
Journal Detail:
Title:  Physical chemistry chemical physics : PCCP     Volume:  9     ISSN:  1463-9076     ISO Abbreviation:  Phys Chem Chem Phys     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-16     Completed Date:  2008-02-29     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  100888160     Medline TA:  Phys Chem Chem Phys     Country:  England    
Other Details:
Languages:  eng     Pagination:  5966-74     Citation Subset:  IM    
Department of Chemistry, The University of Western Ontario, London, ON, Canada.
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MeSH Terms
Biosensing Techniques / methods*
COS Cells / metabolism,  ultrastructure
Cell Physiological Phenomena*
Cercopithecus aethiops
Ethanol / pharmacology
Microscopy, Electron, Scanning / methods
Tetradecanoylphorbol Acetate / pharmacology
Reg. No./Substance:
16561-29-8/Tetradecanoylphorbol Acetate; 64-17-5/Ethanol

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