| Interrogation of living cells using alternating current scanning electrochemical microscopy (AC-SECM). | |
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MedLine Citation:
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PMID: 18004408 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this paper we present the application of alternating current scanning electrochemical microscopy (AC-SECM) to the study of living cells. Commercial AFM instrumentation was modified to allow for performing robust AC-SECM measurements. Constant height AC imaging of the Cos-7 cells, performed directly in cell culture medium without the addition of a redox mediator, provided topographical information of the cell. Stationary tip measurements on the AC current were carried out to investigate the cellular activity of a single cell. The dependence of AC current magnitude on tip-to-sample separation distance was used to monitor real time changes in cell height of individual Cos-7 cells. Furthermore, AC-SECM was employed to observe changes in metabolic cellular activity stimulated by ethanol and phorbol-1,2-myristate-acetate-3. The effect of changing cellular activity on constant height AC-SECM imaging was also studied. |
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Authors:
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Piotr M Diakowski; Zhifeng Ding |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-10-09 |
Journal Detail:
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Title: Physical chemistry chemical physics : PCCP Volume: 9 ISSN: 1463-9076 ISO Abbreviation: Phys Chem Chem Phys Publication Date: 2007 Dec |
Date Detail:
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Created Date: 2007-11-16 Completed Date: 2008-02-29 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 100888160 Medline TA: Phys Chem Chem Phys Country: England |
Other Details:
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Languages: eng Pagination: 5966-74 Citation Subset: IM |
Affiliation:
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Department of Chemistry, The University of Western Ontario, London, ON, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biosensing Techniques / methods* COS Cells / metabolism, ultrastructure Cell Physiological Phenomena* Cercopithecus aethiops Electrochemistry Ethanol / pharmacology Microscopy, Electron, Scanning / methods Tetradecanoylphorbol Acetate / pharmacology |
| Chemical | |
Reg. No./Substance:
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16561-29-8/Tetradecanoylphorbol Acetate; 64-17-5/Ethanol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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