Document Detail

Interrogating cell signalling network sensitively monitors cell fate transition during early differentiation of mouse embryonic stem cells.
MedLine Citation:
PMID:  20596958     Owner:  NLM     Status:  MEDLINE    
The different cell types in an animal are often considered to be specified by combinations of transcription factors, and defined by marker gene expression. This paradigm is challenged, however, in stem cell research and application. Using a mouse embryonic stem cell (mESC) culture system, here we show that the expression level of many key stem cell marker genes/transcription factors such as Oct4, Sox2 and Nanog failed to monitor cell status transition during mESC differentiation. On the other hand, the response patterns of cell signalling network to external stimuli, as monitored by the dynamics of protein phosphorylation, changed dramatically. Our results also suggest that an irreversible alternation in cell signalling network precedes the adjustment of transcription factor levels. This is consistent with the notion that signal transduction events regulate cell fate specification. We propose that interrogating cell signalling network can assess the cell property more precisely, and provide a sensitive measurement for the early events in cell fate transition. We wish to bring up attention to the potential problem of cell identification using a few marker genes, and suggest a novel methodology to address this issue.
ZhiCao Yue; FengFeng Zhuang; Yi-Hsin Liu; Chih-ming Ho
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2010-02-12
Journal Detail:
Title:  Science China. Life sciences     Volume:  53     ISSN:  1869-1889     ISO Abbreviation:  Sci China Life Sci     Publication Date:  2010 Jan 
Date Detail:
Created Date:  2010-07-02     Completed Date:  2010-11-02     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101529880     Medline TA:  Sci China Life Sci     Country:  China    
Other Details:
Languages:  eng     Pagination:  78-86     Citation Subset:  IM    
Department of Mechanical and Aerospace Engineering, University of California, Los Angeles, CA 90095, USA.
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MeSH Terms
Blotting, Western
Cell Differentiation / drug effects,  genetics,  physiology*
Cell Line
Embryonic Stem Cells / cytology,  drug effects,  metabolism*
Fibroblast Growth Factor 8 / pharmacology
Flow Cytometry
Gene Expression / drug effects
Green Fluorescent Proteins / genetics,  metabolism
Leukemia Inhibitory Factor / pharmacology
Octamer Transcription Factor-3 / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Signal Transduction / genetics,  physiology*
Time Factors
Transcription Factors / genetics,  metabolism*
Grant Support
Reg. No./Substance:
0/Leukemia Inhibitory Factor; 0/Octamer Transcription Factor-3; 0/Pou5f1 protein, mouse; 0/Transcription Factors; 147336-22-9/Green Fluorescent Proteins; 148997-75-5/Fibroblast Growth Factor 8

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