Document Detail

Interrelationships between neutrophil elastase, serum alpha, -antitrypsin, lung function and chest radiography in patients with chronic airflow obstruction.
MedLine Citation:
PMID:  313728     Owner:  NLM     Status:  MEDLINE    
We assayed protease and elastase activity of lysosomal granules of purified neutrophil suspensions in 58 patients with chronic irreversible airflow obstruction and compared them to 26 healthy control subjects. Denatured hemoglobin and tritiated elastin were used as substrates for protease and elastase assays. Forty-two patients had M antitrypsin phemotype, five had MS, and 11 had Z variant (five were homozygotes and six were heterozygotes). We did not find significant differences in mean lysosomal elastase or protease activity between patients with normal antitrypsin and control subjects; however, a few patients had concentrations of neutrophil elastase that exceeded the range among control subjects. There was no significant correlation between neutrophil protease or elastase activity and age, smoking, degree of airway obstruction, diffusing capacity, lung elastic recoil, or radiologic presence of emphysema in patients with M and MS antitrypsin. In patients with Z variant antitrypsin, protease and elastase concentrations per unit of lysosomal protein were not significantly different from those in control subjects or M patients; however, both elastase and protease content per 108 neurtophils was significantly higher in homozygous and heterozygous Z patients as compared to normalsubjects and M patients, which suggest an increase in the neutrophil content of protease and elastase in patients with Z antitrypsin deficiency. These results suggest that hte concentrations of protease and elastase in neutrophils do not appear to interact as additive risk factors in the pulmonary impairment of most patients with chronic airflow obstruction, but may be of importance as risk factors in patients with Z or MZ phenotype and in a few patients with M phenotype.
R T Abboud; J M Rushton; S Grzybowski
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  The American review of respiratory disease     Volume:  120     ISSN:  0003-0805     ISO Abbreviation:  Am. Rev. Respir. Dis.     Publication Date:  1979 Jul 
Date Detail:
Created Date:  1979-09-17     Completed Date:  1979-09-17     Revised Date:  2004-11-17    
Medline Journal Info:
Nlm Unique ID:  0370523     Medline TA:  Am Rev Respir Dis     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  31-40     Citation Subset:  AIM; IM    
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MeSH Terms
Forced Expiratory Volume
Lung Compliance
Lung Diseases, Obstructive / enzymology*,  physiopathology,  radiography
Middle Aged
Neutrophils / enzymology*
Pancreatic Elastase / blood*
Peptide Hydrolases / blood
Pulmonary Diffusing Capacity
Pulmonary Emphysema / enzymology,  physiopathology,  radiography
Smoking / complications,  physiopathology
Total Lung Capacity
alpha 1-Antitrypsin Deficiency*
Reg. No./Substance:
EC 3.4.-/Peptide Hydrolases; EC Elastase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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