| Interrelation of intracellular proteases with total parenteral nutrition-induced gut mucosal atrophy and increase of mucosal macromolecular transmission in rats. | |
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MedLine Citation:
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PMID: 8551645 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Total parenteral nutrition (TPN) is known to induce mucosal atrophy and to increase macromolecular transmission of the small intestine. The potential participation of various proteases in that process was investigated. Male Wistar rats were randomly divided into two groups: the TPN group (n = 11) received a standard TPN (250 kcal/kg per day, 1.78 g nitrogen/kg per day) and the FED group (n = 10) received a standard rat food for 1 week. This was followed by an examination of gut macromolecular transmission of fluorescein isothiocyanate dextran 70,000 (FITC-dextran) after intragastric injection and of the activities of gut mucosal cathepsins B, H, and L and of proteasome. Mucosal wet weight and protein content decreased significantly by TPN for 1 week. In both groups, the activities of all proteases in the ileum were significantly greater than in the jejunum. In the TPN group, cathepsin L and H activities in the ileum, and cathepsin B activity in both the jejunum and the ileum, were greater than those in the FED group. The portal concentration of FITC-dextran was higher than arterial and venous concentrations in the both groups. In the TPN group, the portal FITC-dextran concentration increased significantly compared with the FED group. In conclusion, active proteolysis is not associated with TPN-induced mucosal atrophy. Cathepsins activities in the ileum increase as a result of TPN. Interrelationship is implicated between increase of lysosomal protease activity and the deterioration of the intestinal barrier function, which permits macromolecular transmission. |
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Authors:
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M Kishibuchi; T Tsujinaka; S Iijima; M Yano; C Ebisui; K Kan; T Morimoto; T Mori |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: JPEN. Journal of parenteral and enteral nutrition Volume: 19 ISSN: 0148-6071 ISO Abbreviation: JPEN J Parenter Enteral Nutr Publication Date: 1995 May-Jun |
Date Detail:
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Created Date: 1996-02-20 Completed Date: 1996-02-20 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 7804134 Medline TA: JPEN J Parenter Enteral Nutr Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 187-92 Citation Subset: IM |
Affiliation:
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Department of Surgery II, Osaka University Medical School, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Atrophy Cathepsin B / metabolism Cathepsin H Cathepsin L Cathepsins / metabolism Cysteine Endopeptidases / metabolism Dextrans / metabolism* Endopeptidases / metabolism* Fluorescein-5-isothiocyanate / analogs & derivatives*, metabolism Ileum / enzymology Intestinal Mucosa / metabolism*, pathology* Intestine, Small / metabolism, pathology Jejunum / enzymology Male Multienzyme Complexes / metabolism Parenteral Nutrition, Total / adverse effects* Proteasome Endopeptidase Complex Rats Rats, Wistar |
| Chemical | |
Reg. No./Substance:
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0/Multienzyme Complexes; 0/fluorescein isothiocyanate dextran; 3326-32-7/Fluorescein-5-isothiocyanate; 9004-54-0/Dextrans; EC 3.4.-/Cathepsins; EC 3.4.-/Endopeptidases; EC 3.4.22.-/Cysteine Endopeptidases; EC 3.4.22.1/Cathepsin B; EC 3.4.22.15/Cathepsin L; EC 3.4.22.15/Ctsl protein, rat; EC 3.4.22.16/Cathepsin H; EC 3.4.22.16/Ctsh protein, rat; EC 3.4.25.1/Proteasome Endopeptidase Complex |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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