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Interrelated lipid alterations and their influence on the proliferation and fusion of cultured myogenic cells.
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MedLine Citation:
PMID:  565779     Owner:  NLM     Status:  MEDLINE    
We have cultured myogenic cells derived from primary explants and a cell line (L6) in a lipid-depleted medium (LDM) and produced large alterations of the fatty acyl and polar headgroup composition and of the cellular sterol levels. These alterations were produced by altering the composition of the media as follows: removing biotin and providing exogenous fatty acid; removing choline and providing exogenous ethanolamine or choline analogues; and by adding 25-OH cholesterol, an inhibitor of 3-hydroxy-3-methylglutarate (HMG)-CoA reductase. Relatively small, secondary alterations of other lipid classes accompany the large primary alteration. In general, they are not obviously compensatory for the primary alteration by retaining some physical property. We have explored the influence of these lipid alterations on myoblast proliferation and fusion into myotubes. In general, considerable variability appears tolerated, but there also appear to be limits. Long-term cultures grown in media containing a single fatty acid do not proliferate indefinitely, and the fatty acid does not become the sole fatty acyl component of the phospholipids. This phenomenon is also observed for cultures enriched in phosphatidylethanolamine (PE) or phosphatidyldimethylethanolamine (PDME). The influence of the lipid alterations on fusion is particularly interesting. The inclusion of 25-OH cholesterol inhibits fusion. Enrichment of the fatty acyl chains with elaidate or the polar headgroups with PE also inhibits fusion, but in contrast to that by 25-OH cholesterol, a significant fraction of the myoblasts are aligned and interacting with each other. Oleate enrichment enhances the rate of fusion.
A F Horwitz; A Wight; P Ludwig; R Cornell
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of cell biology     Volume:  77     ISSN:  0021-9525     ISO Abbreviation:  J. Cell Biol.     Publication Date:  1978 May 
Date Detail:
Created Date:  1978-07-24     Completed Date:  1978-07-24     Revised Date:  2010-09-02    
Medline Journal Info:
Nlm Unique ID:  0375356     Medline TA:  J Cell Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  334-57     Citation Subset:  IM    
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MeSH Terms
Biotin / metabolism
Cell Division*
Cell Fusion*
Cell Line
Chick Embryo
Cholesterol / metabolism
Choline / metabolism
Fatty Acids / metabolism
Membrane Lipids / metabolism*
Phospholipids / biosynthesis
Sterols / biosynthesis
Reg. No./Substance:
0/Fatty Acids; 0/Membrane Lipids; 0/Phospholipids; 0/Sterols; 57-88-5/Cholesterol; 58-85-5/Biotin; 62-49-7/Choline

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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Journal Information
Journal ID (nlm-ta): J Cell Biol
ISSN: 0021-9525
ISSN: 1540-8140
Publisher: The Rockefeller University Press
Article Information
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Print publication date: Day: 1 Month: 5 Year: 1978
Volume: 77 Issue: 2
First Page: 334 Last Page: 357
ID: 2110055
Publisher Id: 78171635
PubMed Id: 565779

Interrelated lipid alterations and their influence on the proliferation and fusion of cultured myogenic cells

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