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Interpopulation patterns of divergence and selection across the transcriptome of the copepod Tigriopus californicus.
MedLine Citation:
PMID:  21199025     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
The accumulation of genetic incompatibilities between isolated populations is thought to lead to the evolution of intrinsic postzygotic isolation. The molecular basis for these mechanisms, however, remains poorly understood. The intertidal copepod Tigriopus californicus provides unique opportunities for addressing mechanistic questions regarding the early stages of speciation; hybrids between highly divergent populations are fertile and viable, but exhibit reduced fitness at the F(2) or later generations. Given the current scarcity of genomic information in taxa at incipient stages of reproductive isolation, we utilize high-throughout 454 pyrosequencing to characterize a substantial fraction of protein-coding regions (the transcriptome) of T. californicus. Our sequencing effort was divided equally between two divergent populations in order to estimate levels of divergence and to reveal patterns of selection across the transcriptome. Assembly of sequences generated over 40 000 putatively unique transcripts (unigenes) for each population, 19 622 of which were orthologous between populations. BLAST searches of public databases determined protein identity and functional features for 15 402 and 12 670 unigenes, respectively. Based on rates of nonsynonymous and synonymous substitutions in 5897 interpopulation orthologs (those >150 bp and with at least 2X coverage), we identified 229 potential targets of positive selection. Many of these genes are predicted to be involved in several metabolic processes, and to function in hydrolase, peptidase and binding activities. The library of T. californicus coding regions, annotated with their predicted functions and level of divergence, will serve as an invaluable resource for elucidating molecular mechanisms underlying the early stages of speciation.
Authors:
Felipe S Barreto; Gary W Moy; Ronald S Burton
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Publication Detail:
Type:  Journal Article     Date:  2010-12-24
Journal Detail:
Title:  Molecular ecology     Volume:  20     ISSN:  1365-294X     ISO Abbreviation:  Mol. Ecol.     Publication Date:  2011 Feb 
Date Detail:
Created Date:  2011-01-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9214478     Medline TA:  Mol Ecol     Country:  England    
Other Details:
Languages:  eng     Pagination:  560-72     Citation Subset:  IM    
Copyright Information:
© 2010 Blackwell Publishing Ltd.
Affiliation:
Marine Biology Research Division, Scripps Institution of Oceanography, University of California, San Diego, La Jolla, CA 92037, USA.
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