| Interplay of pro- and anti-inflammatory cytokines to determine lipid accretion in adipocytes. | |
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MedLine Citation:
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PMID: 23381555 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Objective:Obesity is associated with an increase in various pro-inflammatory and anti-inflammatory cytokines, but the interplay of these cytokines is incompletely understood. We conducted experiments to test a broader hypothesis that a dynamic interplay of pro-inflammatory and anti-inflammatory cytokines controls lipid storage in adipocytes.Design:Three experiments were designed to test the overall hypothesis that proinflammatory cytokine (for example, tumor necrosis factor-α (TNF-α) inhibits anti-inflammatory cytokine (for example, adiponectin) activity in an attempt to limit excess lipid accumulation in adipocytes.Results:Experiment one showed that in pro-inflammatory animal models (ap2-P65, ob/ob and high-fat diet-induced obese mice), the increase in TNF-α expression was associated with a decrease in adiponectin expression. Experiment two showed that in 3T3-L1 adipocytes, TNF-α significantly reduced lipid accumulation and glucose uptake induced by adiponectin, and increased lipolysis. Experiment three showed that in 3T3-L1 adipocytes, TNF-α reduced mRNA and protein expression of adiponectin. Adiponectin gene transcription and mRNA stability were both reduced by TNF-α. The expression of peroxisome proliferator-activated receptor gamma, an activator of adiponectin gene promoter, was reduced by TNF-α. The inhibitory activity of TNF-α was blocked by the chemical inhibitors of NF-κB and super suppressor IκBα.Conclusions:TNF-α opposes the action of adiponectin in the regulation of lipid metabolism, and inhibits adiponectin expression at transcriptional and post-transcriptional levels. The results suggest that pro-inflammatory cytokine inhibit anti-inflammatory cytokine in adipocytes to reduce lipid storage. This suggests a potential role of anti-inflammatory cytokines in the control of adipose tissue expansion.International Journal of Obesity advance online publication, 5 February 2013; doi:10.1038/ijo.2013.9. |
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Authors:
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Y Wang; H Wang; V Hegde; O Dubuisson; Z Gao; N V Dhurandhar; J Ye |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-2-05 |
Journal Detail:
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Title: International journal of obesity (2005) Volume: - ISSN: 1476-5497 ISO Abbreviation: Int J Obes (Lond) Publication Date: 2013 Feb |
Date Detail:
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Created Date: 2013-2-5 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101256108 Medline TA: Int J Obes (Lond) Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Antioxidant and Gene Regulation Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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