Document Detail


Interplay of pro- and anti-inflammatory cytokines to determine lipid accretion in adipocytes.
MedLine Citation:
PMID:  23381555     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Objective:Obesity is associated with an increase in various pro-inflammatory and anti-inflammatory cytokines, but the interplay of these cytokines is incompletely understood. We conducted experiments to test a broader hypothesis that a dynamic interplay of pro-inflammatory and anti-inflammatory cytokines controls lipid storage in adipocytes.Design:Three experiments were designed to test the overall hypothesis that proinflammatory cytokine (for example, tumor necrosis factor-α (TNF-α) inhibits anti-inflammatory cytokine (for example, adiponectin) activity in an attempt to limit excess lipid accumulation in adipocytes.Results:Experiment one showed that in pro-inflammatory animal models (ap2-P65, ob/ob and high-fat diet-induced obese mice), the increase in TNF-α expression was associated with a decrease in adiponectin expression. Experiment two showed that in 3T3-L1 adipocytes, TNF-α significantly reduced lipid accumulation and glucose uptake induced by adiponectin, and increased lipolysis. Experiment three showed that in 3T3-L1 adipocytes, TNF-α reduced mRNA and protein expression of adiponectin. Adiponectin gene transcription and mRNA stability were both reduced by TNF-α. The expression of peroxisome proliferator-activated receptor gamma, an activator of adiponectin gene promoter, was reduced by TNF-α. The inhibitory activity of TNF-α was blocked by the chemical inhibitors of NF-κB and super suppressor IκBα.Conclusions:TNF-α opposes the action of adiponectin in the regulation of lipid metabolism, and inhibits adiponectin expression at transcriptional and post-transcriptional levels. The results suggest that pro-inflammatory cytokine inhibit anti-inflammatory cytokine in adipocytes to reduce lipid storage. This suggests a potential role of anti-inflammatory cytokines in the control of adipose tissue expansion.International Journal of Obesity advance online publication, 5 February 2013; doi:10.1038/ijo.2013.9.
Authors:
Y Wang; H Wang; V Hegde; O Dubuisson; Z Gao; N V Dhurandhar; J Ye
Related Documents :
24151515 - Recent advances in the pathogenesis of autoimmune hair loss disease alopecia areata.
12882915 - Free radicals and the pathogenesis of type 1 diabetes: beta-cell cytokine-mediated free...
24192715 - Targeting pkc in human t cells using sotrastaurin (aeb071) preserves regulatory t cells...
24614605 - Osteopathic manipulative therapy induces early plasma cytokine release and mobilization...
24151515 - Recent advances in the pathogenesis of autoimmune hair loss disease alopecia areata.
21709155 - A critical role for granzymes in antigen cross-presentation through regulating phagocyt...
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-2-05
Journal Detail:
Title:  International journal of obesity (2005)     Volume:  -     ISSN:  1476-5497     ISO Abbreviation:  Int J Obes (Lond)     Publication Date:  2013 Feb 
Date Detail:
Created Date:  2013-2-5     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101256108     Medline TA:  Int J Obes (Lond)     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Antioxidant and Gene Regulation Laboratory, Pennington Biomedical Research Center, Louisiana State University System, Baton Rouge, LA, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Mild ovarian stimulation for in vitro fertilization: one perspective from the USA.
Next Document:  Genetic association analysis of vitamin D pathway with obesity traits.