Document Detail


Interplay between manganese and zinc homeostasis in the human pathogen Streptococcus pneumoniae.
MedLine Citation:
PMID:  21275153     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
ICP-MS analysis of Streptococcus pneumoniae reveals a high cell-associated Mn(II) concentration that is comparable to that of Zn(II). Stressing these cells with 100–200 μM Zn(II) leads to a slow-growth phenotype and a total Mn(II) concentration that is reduced, with no decrease of other metal ions. Supplementation of the growth media with as little as 10 μM Mn(II) fully restores the growth defect and cell-associated Mn(II) to normal levels. DNA microarray analysis reveals that zinc stress induces the expected upregulation of czcD (encoding a zinc effluxer), but also a pleiotropic transcriptional response suggestive of mild cell wall stress. Genes encoding a nitric oxide (NO) detoxification system (nmlR) and the Mn(II) uptake system (psaBCA) are also induced. We conclude that Zn(II) toxicity results in a cytoplasmic Mn(II) deficiency, possibly caused by competition at the Mn(II) uptake transporter protein PsaA.
Authors:
Faith E Jacobsen; Krystyna M Kazmierczak; John P Lisher; Malcolm E Winkler; David P Giedroc
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Metallomics : integrated biometal science     Volume:  3     ISSN:  1756-591X     ISO Abbreviation:  Metallomics     Publication Date:  2011 Jan 
Date Detail:
Created Date:  2011-01-27     Completed Date:  2011-05-10     Revised Date:  2013-06-30    
Medline Journal Info:
Nlm Unique ID:  101478346     Medline TA:  Metallomics     Country:  England    
Other Details:
Languages:  eng     Pagination:  38-41     Citation Subset:  IM    
Affiliation:
Department of Chemistry, Indiana University, Bloomington, IN 47405-7102, USA.
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MeSH Terms
Descriptor/Qualifier:
Adhesins, Bacterial / genetics,  metabolism
Cytological Techniques
DNA / metabolism
DNA-Binding Proteins / genetics,  metabolism
Gene Expression Profiling
Homeostasis / physiology
Lipoproteins / genetics,  metabolism
Manganese / metabolism*
Mass Spectrometry
Membrane Transport Proteins / genetics,  metabolism
Oligonucleotide Array Sequence Analysis
Repressor Proteins / genetics,  metabolism
Streptococcus pneumoniae / metabolism*
Up-Regulation
Zinc / metabolism*
Grant Support
ID/Acronym/Agency:
AI060744/AI/NIAID NIH HHS; F32 AI084445/AI/NIAID NIH HHS; F32 AI084445-01/AI/NIAID NIH HHS; F32 AI084445-02/AI/NIAID NIH HHS; GM042569/GM/NIGMS NIH HHS; R01 AI060744-04/AI/NIAID NIH HHS; R01 AI060744-05/AI/NIAID NIH HHS; R01 GM042569-21/GM/NIGMS NIH HHS; R01 GM042569-22/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Adhesins, Bacterial; 0/DNA-Binding Proteins; 0/Lipoproteins; 0/Membrane Transport Proteins; 0/PsaA protein, Streptococcus; 0/Repressor Proteins; 7439-96-5/Manganese; 7440-66-6/Zinc; 9007-49-2/DNA
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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