| Interplay between manganese and zinc homeostasis in the human pathogen Streptococcus pneumoniae. | |
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MedLine Citation:
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PMID: 21275153 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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ICP-MS analysis of Streptococcus pneumoniae reveals a high cell-associated Mn(II) concentration that is comparable to that of Zn(II). Stressing these cells with 100–200 μM Zn(II) leads to a slow-growth phenotype and a total Mn(II) concentration that is reduced, with no decrease of other metal ions. Supplementation of the growth media with as little as 10 μM Mn(II) fully restores the growth defect and cell-associated Mn(II) to normal levels. DNA microarray analysis reveals that zinc stress induces the expected upregulation of czcD (encoding a zinc effluxer), but also a pleiotropic transcriptional response suggestive of mild cell wall stress. Genes encoding a nitric oxide (NO) detoxification system (nmlR) and the Mn(II) uptake system (psaBCA) are also induced. We conclude that Zn(II) toxicity results in a cytoplasmic Mn(II) deficiency, possibly caused by competition at the Mn(II) uptake transporter protein PsaA. |
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Authors:
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Faith E Jacobsen; Krystyna M Kazmierczak; John P Lisher; Malcolm E Winkler; David P Giedroc |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Metallomics : integrated biometal science Volume: 3 ISSN: 1756-591X ISO Abbreviation: Metallomics Publication Date: 2011 Jan |
Date Detail:
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Created Date: 2011-01-27 Completed Date: 2011-05-10 Revised Date: 2013-05-27 |
Medline Journal Info:
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Nlm Unique ID: 101478346 Medline TA: Metallomics Country: England |
Other Details:
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Languages: eng Pagination: 38-41 Citation Subset: IM |
Affiliation:
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Department of Chemistry, Indiana University, Bloomington, IN 47405-7102, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adhesins, Bacterial
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genetics,
metabolism Cytological Techniques DNA / metabolism DNA-Binding Proteins / genetics, metabolism Gene Expression Profiling Homeostasis / physiology Lipoproteins / genetics, metabolism Manganese / metabolism* Mass Spectrometry Membrane Transport Proteins / genetics, metabolism Oligonucleotide Array Sequence Analysis Repressor Proteins / genetics, metabolism Streptococcus pneumoniae / metabolism* Up-Regulation Zinc / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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AI060744/AI/NIAID NIH HHS; F32 AI084445/AI/NIAID NIH HHS; F32 AI084445-01/AI/NIAID NIH HHS; F32 AI084445-02/AI/NIAID NIH HHS; GM042569/GM/NIGMS NIH HHS; R01 AI060744-04/AI/NIAID NIH HHS; R01 AI060744-05/AI/NIAID NIH HHS; R01 GM042569-21/GM/NIGMS NIH HHS; R01 GM042569-22/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Adhesins, Bacterial; 0/DNA-Binding Proteins; 0/Lipoproteins; 0/Membrane Transport Proteins; 0/PsaA protein, Streptococcus; 0/Repressor Proteins; 7439-96-5/Manganese; 7440-66-6/Zinc; 9007-49-2/DNA |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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