| Interplay between liquid crystalline and isotropic gels in self-assembled neurofilament networks. | |
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MedLine Citation:
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PMID: 18583309 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Neurofilaments (NFs) are a major constituent of nerve cell axons that assemble from three subunit proteins of low (NF-L), medium (NF-M), and high (NF-H) molecular weight into a 10 nm diameter rod with radiating sidearms to form a bottle-brush-like structure. Here, we reassemble NFs in vitro from varying weight ratios of the subunit proteins, purified from bovine spinal cord, to form homopolymers of NF-L or filaments composed of NF-L and NF-M (NF-LM), NF-L and NF-H (NF-LH), or all three subunits (NF-LMH). At high protein concentrations, NFs align to form a nematic liquid crystalline gel with a well-defined spacing determined with synchrotron small angle x-ray scattering. Near physiological conditions (86 mM monovalent salt and pH 6.8), NF-LM networks with a high NF-M grafting density favor nematic ordering whereas filaments composed of NF-LH transition to an isotropic gel at low protein concentrations as a function of increasing mole fraction of NF-H subunits. The interfilament distance decreases with NF-M grafting density, opposite the trend seen with NF-LH networks. This suggests a competition between the more attractive NF-M sidearms, forming a compact aligned nematic gel, and the repulsive NF-H sidearms, favoring a more expansive isotropic gel, at 86 mM monovalent salt. These interactions are highly salt dependent and the nematic gel phase is stabilized with increasing monovalent salt. |
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Authors:
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Jayna B Jones; Cyrus R Safinya |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Biophysical journal Volume: 95 ISSN: 1542-0086 ISO Abbreviation: Biophys. J. Publication Date: 2008 Jul |
Date Detail:
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Created Date: 2008-06-27 Completed Date: 2008-07-25 Revised Date: 2010-09-22 |
Medline Journal Info:
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Nlm Unique ID: 0370626 Medline TA: Biophys J Country: United States |
Other Details:
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Languages: eng Pagination: 823-35 Citation Subset: IM |
Affiliation:
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Materials Department, University of California, Santa Barbara, California 93106, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Anisotropy Gels / chemistry* Liquid Crystals / chemistry* Multiprotein Complexes / chemistry Neurofilament Proteins / chemistry* Scattering, Small Angle X-Ray Diffraction |
| Chemical | |
Reg. No./Substance:
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0/Gels; 0/Multiprotein Complexes; 0/Neurofilament Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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