Document Detail


Interplay between MAMP-triggered and SA-mediated defense responses.
MedLine Citation:
PMID:  18005228     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Plants respond to pathogen infection using an innate immune system with at least two distinct recognition mechanisms. One mechanism recognizes microbe-associated molecular patterns (MAMPs). The other is based on resistance (R) genes and specifically recognizes certain pathogen virulence factors, including those delivered through the type III secretion system (TTSS) of bacteria. Salicylic acid (SA)-mediated responses are an important part of the R gene-mediated defense. Substantial overlaps between MAMP-triggered and SA-mediated responses have been reported. However, interactions between MAMP-triggered and SA-mediated signaling mechanisms have not been well documented. Here we report intimate interactions between MAMP-triggered and SA-mediated signaling. We found that SA accumulated at a higher level 6 h after treatment with a MAMP, flg22 or inoculation with Pseudomonas syringae pv. tomato DC3000 (PstDC3000) hrcC mutant, which is deficient in TTSS function. Disruptions of SA signaling components, such as SID2 and PAD4, strongly affected MAMP-triggered responses monitored by expression profiling. We found two groups of genes that were induced by PstDC3000 hrcC in an SA-dependent manner. One group was SID2-dependent at all time points, whereas the other was SID2-independent at early time points and SID2-dependent at later time points. Thus, the expression of the latter genes responds to MAMPs through both SA-independent and SA-dependent signaling mechanisms. Strong resistance to PstDC3000 hrcC was dependent on SA signaling. These results indicate that the SA increase triggered by MAMPs is a major component of the MAMP-triggered signaling mechanism, explaining the overlapping spectra of MAMP-triggered and SA-mediated responses.
Authors:
Kenichi Tsuda; Masanao Sato; Jane Glazebrook; Jerry D Cohen; Fumiaki Katagiri
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.     Date:  2007-11-14
Journal Detail:
Title:  The Plant journal : for cell and molecular biology     Volume:  53     ISSN:  1365-313X     ISO Abbreviation:  Plant J.     Publication Date:  2008 Mar 
Date Detail:
Created Date:  2008-02-27     Completed Date:  2008-05-20     Revised Date:  2008-11-05    
Medline Journal Info:
Nlm Unique ID:  9207397     Medline TA:  Plant J     Country:  England    
Other Details:
Languages:  eng     Pagination:  763-75     Citation Subset:  IM    
Affiliation:
Department of Plant Biology, Microbial and Plant Genomics Institute, University of Minnesota, 1500 Gortner Avenue, St Paul, MN 55108, USA.
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MeSH Terms
Descriptor/Qualifier:
Arabidopsis / genetics,  metabolism*,  microbiology*
Gene Expression Profiling
Gene Expression Regulation, Plant / physiology*
Intramolecular Transferases / genetics,  metabolism
Plant Diseases / immunology
Protein Array Analysis
Pseudomonas syringae / physiology*
RNA, Messenger / genetics,  metabolism
RNA, Plant / genetics,  metabolism
Salicylic Acid / metabolism*
Chemical
Reg. No./Substance:
0/RNA, Messenger; 0/RNA, Plant; 69-72-7/Salicylic Acid; EC 5.4.-/Intramolecular Transferases; EC 5.4.4.2/isochorismate synthase
Comments/Corrections
Erratum In:
Plant J. 2008 Sep;55(6):1061

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