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Interplay Of Sugar, Light and Gibberellins In Expression Of Rosa hybrida Vacuolar Invertase 1 Regulation.
MedLine Citation:
PMID:  25108242     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Our previous findings showed that the expression of RhVI1 was tightly correlated with the ability of bud to grow out and was under sugar, gibberellins and light control. Here, we aimed to give an insight into the mechanistic basis of this regulation. In situ hybridization showed that RhVI1 expression was localized in epidermal cells of young leaf of bursting bud. Thereafter, we isolated a 895bp fragment of the promoter of RhVI1. In silico analysis identified putative cis-elements involved in the response to sugars, light and gibberellins on its proximal part (595bp). To carry out functional analysis of the RhVI1 promoter in a homologous system, we developed a direct method for stable transformation of rose cells. 5' deletions of the proximal promoter fused to the uidA reporter gene were inserted into the rose cell genome to study cells response to exogenous and endogenous stimuli. Deletion analysis revealed that the 468bp promoter fragment is sufficient to trigger reporter gene activity in response to light, sugars and gibberellins. This region confers sucrose and fructose, but not glucose, responsive-activation in the dark. Inversely, the -595bp to -468bp region that carries the sugar-repressive element (SRE) is required to down-regulate the RhVI1 promoter in response to sucrose and fructose in the dark. We also demonstrate that sugar/light and gibberellin/light act synergistically to sharply upregulate GUS activity under the control of the 595bp pRhVI1 region. These results reveal that the 127bp promoter fragment located between -595pb and -468pb is critical for light-and-sugar and light-and-gibberellins to act synergistically.
Authors:
Amélie Rabot; Virginie Portemer; Thomas Péron; Eric Mortreau; Nathalie Leduc; Latifa Hamama; Pierre Coutos-Thévenot; Rossitza Atanassova; Soulaiman Sakr; José Le Gourrierec
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-8-9
Journal Detail:
Title:  Plant & cell physiology     Volume:  -     ISSN:  1471-9053     ISO Abbreviation:  Plant Cell Physiol.     Publication Date:  2014 Aug 
Date Detail:
Created Date:  2014-8-9     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9430925     Medline TA:  Plant Cell Physiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
© The Author 2014. Published by Oxford University Press on behalf of Japanese Society of Plant Physiologists. All rights reserved. For Permissions, please e-mail: journals.permissions@oup.com.
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