Document Detail

Interplay of macrophages and T cells in the lung vasculature.
MedLine Citation:
PMID:  22387295     Owner:  NLM     Status:  MEDLINE    
In severe pulmonary arterial hypertension (PAH), vascular lesions are composed of phenotypically altered vascular and inflammatory cells that form clusters or tumorlets. Because macrophages are found in increased numbers in intravascular and perivascular space in human PAH, here we address the question whether macrophages play a role in pulmonary vascular remodeling and whether accumulation of macrophages in the lung vasculature could be compromised by the immune system. We used the mouse macrophage cell line RAW 264.7 because these cells are resistant to apoptosis, have high proliferative capacity, and resemble cells in the plexiform lesions that tend to pile up instead of maintaining a monolayer. Cells were characterized by immunocytochemistry with cell surface markers (Lycopersicon Esculentum Lectin, CD117, CD133, FVIII, CD31, VEGFR-2, and S100). Activated, but not quiescent, T cells were able to suppress RAW 264.7 cell proliferative and migration activity in vitro. The carboxyfluorescein diacetate-labeled RAW 264.7 cells were injected into the naïve Sprague Dawley (SD) rat and athymic nude rat. Twelve days later, cells were found in the lung vasculature of athymic nude rats that lack functional T cells, contributing to vascular remodeling. No labeled RAW 264.7 cells were detected in the lungs of immune-competent SD rats. Our data demonstrate that T cells can inhibit in vitro migration and in vivo accumulation of macrophage-like cells.
Evgenia Gerasimovskaya; Adelheid Kratzer; Asya Sidiakova; Jonas Salys; Martin Zamora; Laimute Taraseviciene-Stewart
Related Documents :
3652965 - Cell polarity during wound healing in an insect epidermis.
22582115 - Activity and inhibition of prostasin and matriptase on apical and basolateral surfaces ...
2923795 - A quantitative assessment of langerhans cells in oral mucosal lichen planus and leukopl...
6572185 - Large mononuclear ia-positive veiled cells in peyer's patches. i. isolation and charact...
18422505 - Anaplastic and aggressive subcutaneous sarcoma in a seven-month-old dog.
25181625 - Overexpression of myosin is associated with the development of uterine myoma.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-03-02
Journal Detail:
Title:  American journal of physiology. Lung cellular and molecular physiology     Volume:  302     ISSN:  1522-1504     ISO Abbreviation:  Am. J. Physiol. Lung Cell Mol. Physiol.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-16     Completed Date:  2012-07-05     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  100901229     Medline TA:  Am J Physiol Lung Cell Mol Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  L1014-22     Citation Subset:  IM    
Department of Pediatrics, University of Colorado Denver, 12700 E. 19th Ave, Aurora, CO 80045, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Biological Markers / analysis
Cell Communication / immunology*
Cell Line
Cell Movement / immunology
Cell Proliferation
Fluorescent Dyes
Hypertension, Pulmonary / immunology,  pathology,  physiopathology
Lung / blood supply*,  cytology,  immunology
Macrophages / cytology*,  immunology,  transplantation
Models, Biological
Pulmonary Artery / cytology*,  immunology
Rats, Nude
Rats, Sprague-Dawley
T-Lymphocytes / cytology*,  immunology
Grant Support
Reg. No./Substance:
0/Biological Markers; 0/Fluoresceins; 0/Fluorescent Dyes; 0/carboxyfluoresceindiacetate

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Kinase-dependent activation of voltage-gated Ca2+ channels by ET-1 in pulmonary arterial myocytes du...
Next Document:  Analysis of IgG subclasses (IgG1 and IgG3) to recombinant SAG2A protein from Toxoplasma gondii in se...