Document Detail


Interplay of macrophages and T cells in the lung vasculature.
MedLine Citation:
PMID:  22387295     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In severe pulmonary arterial hypertension (PAH), vascular lesions are composed of phenotypically altered vascular and inflammatory cells that form clusters or tumorlets. Because macrophages are found in increased numbers in intravascular and perivascular space in human PAH, here we address the question whether macrophages play a role in pulmonary vascular remodeling and whether accumulation of macrophages in the lung vasculature could be compromised by the immune system. We used the mouse macrophage cell line RAW 264.7 because these cells are resistant to apoptosis, have high proliferative capacity, and resemble cells in the plexiform lesions that tend to pile up instead of maintaining a monolayer. Cells were characterized by immunocytochemistry with cell surface markers (Lycopersicon Esculentum Lectin, CD117, CD133, FVIII, CD31, VEGFR-2, and S100). Activated, but not quiescent, T cells were able to suppress RAW 264.7 cell proliferative and migration activity in vitro. The carboxyfluorescein diacetate-labeled RAW 264.7 cells were injected into the naïve Sprague Dawley (SD) rat and athymic nude rat. Twelve days later, cells were found in the lung vasculature of athymic nude rats that lack functional T cells, contributing to vascular remodeling. No labeled RAW 264.7 cells were detected in the lungs of immune-competent SD rats. Our data demonstrate that T cells can inhibit in vitro migration and in vivo accumulation of macrophage-like cells.
Authors:
Evgenia Gerasimovskaya; Adelheid Kratzer; Asya Sidiakova; Jonas Salys; Martin Zamora; Laimute Taraseviciene-Stewart
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2012-03-02
Journal Detail:
Title:  American journal of physiology. Lung cellular and molecular physiology     Volume:  302     ISSN:  1522-1504     ISO Abbreviation:  Am. J. Physiol. Lung Cell Mol. Physiol.     Publication Date:  2012 May 
Date Detail:
Created Date:  2012-05-16     Completed Date:  2012-07-05     Revised Date:  2013-06-26    
Medline Journal Info:
Nlm Unique ID:  100901229     Medline TA:  Am J Physiol Lung Cell Mol Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  L1014-22     Citation Subset:  IM    
Affiliation:
Department of Pediatrics, University of Colorado Denver, 12700 E. 19th Ave, Aurora, CO 80045, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Biological Markers / analysis
Cell Communication / immunology*
Cell Line
Cell Movement / immunology
Cell Proliferation
Fluoresceins
Fluorescent Dyes
Hypertension, Pulmonary / immunology,  pathology,  physiopathology
Immunohistochemistry
Lung / blood supply*,  cytology,  immunology
Macrophages / cytology*,  immunology,  transplantation
Male
Mice
Models, Biological
Pulmonary Artery / cytology*,  immunology
Rats
Rats, Nude
Rats, Sprague-Dawley
T-Lymphocytes / cytology*,  immunology
Grant Support
ID/Acronym/Agency:
R01 HL086783/HL/NHLBI NIH HHS; R01 HL086783/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Biological Markers; 0/Fluoresceins; 0/Fluorescent Dyes; 0/carboxyfluoresceindiacetate
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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