Document Detail


Interplasmid transposition of Drosophila hobo elements in non-drosophilid insects.
MedLine Citation:
PMID:  8041366     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A modified hobo element from Drosophila melanogaster was introduced into embryos of the housefly, Musca domestica (family Muscidae) and the Queensland fruitfly, Bactrocera tryoni (family Tephritidae) to assess its ability to transpose. Hobo was capable of transposition in these species and transposition products had all of the hallmarks of hobo transposition products recovered from D. melanogaster, including the movement only of sequences precisely delimited by the inverted terminal repeats of hobo, the creation of an 8 bp duplication of the insertion site and an absolute requirement for hobo-encoded transposase. Transposition of hobo into the target gene resulted in a non-random distribution of insertion sites, with 10 of 38 independent insertions into the same nucleotide position. The results indicate that hobo can transpose in heterologous species, further demonstrating the similarity of hobo to Ac (Activator) of Zea mays and Tam3 of Antirrhinum majus. Hobo has excellent potential to act as a gene vector or gene tagging agent in nondrosophilid insects.
Authors:
D A O'Brochta; W D Warren; K J Saville; P W Atkinson
Related Documents :
20449796 - Enhancer/suppressor mutator (en/spm)-like transposable elements of cassava (manihot esc...
17134856 - A novel non-coding dna family in caenorhabditis elegans.
2419836 - Structural analysis of type ii variants within the mouse intracisternal a-particle sequ...
16128396 - A novel insertion sequence element, is yen2, as an epidemiological marker for weakly pa...
8366026 - Comparative analysis of flagellin sequences from escherichia coli strains possessing se...
17020806 - Rna polymerase ii gene (rpb2) encoding the second largest protein subunit in phaeosphae...
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Molecular & general genetics : MGG     Volume:  244     ISSN:  0026-8925     ISO Abbreviation:  Mol. Gen. Genet.     Publication Date:  1994 Jul 
Date Detail:
Created Date:  1994-08-25     Completed Date:  1994-08-25     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0125036     Medline TA:  Mol Gen Genet     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  9-14     Citation Subset:  IM    
Affiliation:
University of Maryland Biotechnology Institute, Center for Agricultural Biotechnology, College Park 20742.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
DNA Transposable Elements*
Diptera / genetics*
Drosophila melanogaster / genetics*
Genetic Vectors
Houseflies / genetics*
Molecular Sequence Data
Plasmids
Sequence Homology, Nucleic Acid
Grant Support
ID/Acronym/Agency:
GM48102/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA Transposable Elements

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The NADH dehydrogenase subunit 7 gene is interrupted by four group II introns in the wheat mitochond...
Next Document:  Cerulenin-resistant mutants of Saccharomyces cerevisiae with an altered fatty acid synthase gene.