Document Detail


Interphase cytogenetic analysis in Argentinean B-cell chronic lymphocytic leukemia patients: association of trisomy 12 and del(13q14).
MedLine Citation:
PMID:  14553950     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We have evaluated genomic aberrations by conventional cytogenetics and fluorescence in situ hybridization (FISH) analysis in a series of 57 Argentinean B-cell chronic lymphocytic leukemia (B-CLL) patients. The studies were performed on stimulated peripheral blood lymphocytes. FISH analysis for trisomy 12, 13q14 deletion, and monosomy of TP53 (also known as p53) was performed according to standard protocols. Our results showed 46.3% of patients with clonal chromosomal alterations by conventional cytogenetics and 80.7% by FISH. Trisomy 12 was found in 21.9% of patients by G-banding analysis and in 35% by FISH studies. Allelic loss of 13q14 was observed in 63.2% patients, most of them showing D13S319 and D13S25 deletion; 11% of patients showed TP53 monosomy. Coexistence of trisomy 12 and 13q14 deletion was found in 17.5% of patients. In this group, deletion 13q14 was the prevalent clone, with percentages 25-35% higher than those observed for trisomy 12, suggesting clonal evolution. The coexistence of trisomy 12 with deletion 13q14 was observed in a higher frequency than reported in the literature. A probable adverse prognosis is suggested for this group of patients, likely related to clonal evolution.
Authors:
Christian Chena; Guillermo Arrossagaray; Mariano Scolnik; María F Palacios; Irma Slavutsky
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cancer genetics and cytogenetics     Volume:  146     ISSN:  0165-4608     ISO Abbreviation:  Cancer Genet. Cytogenet.     Publication Date:  2003 Oct 
Date Detail:
Created Date:  2003-10-13     Completed Date:  2003-11-03     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  7909240     Medline TA:  Cancer Genet Cytogenet     Country:  United States    
Other Details:
Languages:  eng     Pagination:  154-60     Citation Subset:  IM    
Affiliation:
Departamento de Genética, Instituto de Investigaciones Hematológicas, Academia Nacional de Medicina, Buenos Aires, Argentina. cpchena@hematologia.anm.edu.ar
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MeSH Terms
Descriptor/Qualifier:
Aged
Argentina
Chromosome Deletion*
Chromosomes, Human, Pair 12*
Chromosomes, Human, Pair 13*
Cytogenetic Analysis
Female
Gene Dosage
Humans
In Situ Hybridization, Fluorescence
Karyotyping
Leukemia, Lymphocytic, Chronic, B-Cell / genetics*
Male
Middle Aged
Trisomy*

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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