Document Detail


Internally controlled, generic real-time PCR for quantification and multiplex real-time PCR with serotype-specific probes for serotyping of dengue virus infections.
MedLine Citation:
PMID:  22312344     Owner:  NLM     Status:  In-Data-Review    
Abstract/OtherAbstract:
Dengue has become a global public health problem and a sensitive diagnostic test for early phase detection can be life saving. An internally controlled, generic real-time PCR was developed and validated by testing serial dilutions of a DENV positive control RNA in the presence of a fixed amount of IC with results showing a good linearity (R(2) = 0.9967) and a LOD of at least 1.95 × 10(4) copies/mL. Application of the generic PCR on 136 patient samples revealed a sensitivity of 95.8% and specificity of 100%. A newly developed multiplex real-time PCR with serotype-specific probes allowed the serotyping of DENV for 80 out of 92 (87%) generic real-time PCR positive patients. Combined these real-time PCRs offer a convenient diagnostic tool for the sensitive and specific quantification of DENV in clinical specimens with the possibility for serotyping.
Authors:
Sandra Menting; Khoa T D Thai; Tran T T Nga; Hoang L Phuong; Paul Klatser; Katja C Wolthers; Tran Q Binh; Peter J de Vries; Marcel Beld
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Publication Detail:
Type:  Journal Article     Date:  2011-12-05
Journal Detail:
Title:  Advances in virology     Volume:  2011     ISSN:  1687-8647     ISO Abbreviation:  Adv Virol     Publication Date:  2011  
Date Detail:
Created Date:  2012-02-07     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  101508613     Medline TA:  Adv Virol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  514681     Citation Subset:  -    
Affiliation:
Royal Tropical Institute, KIT Biomedical Research, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands.
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