| Internally controlled, generic real-time PCR for quantification and multiplex real-time PCR with serotype-specific probes for serotyping of dengue virus infections. | |
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MedLine Citation:
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PMID: 22312344 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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Dengue has become a global public health problem and a sensitive diagnostic test for early phase detection can be life saving. An internally controlled, generic real-time PCR was developed and validated by testing serial dilutions of a DENV positive control RNA in the presence of a fixed amount of IC with results showing a good linearity (R(2) = 0.9967) and a LOD of at least 1.95 × 10(4) copies/mL. Application of the generic PCR on 136 patient samples revealed a sensitivity of 95.8% and specificity of 100%. A newly developed multiplex real-time PCR with serotype-specific probes allowed the serotyping of DENV for 80 out of 92 (87%) generic real-time PCR positive patients. Combined these real-time PCRs offer a convenient diagnostic tool for the sensitive and specific quantification of DENV in clinical specimens with the possibility for serotyping. |
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Authors:
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Sandra Menting; Khoa T D Thai; Tran T T Nga; Hoang L Phuong; Paul Klatser; Katja C Wolthers; Tran Q Binh; Peter J de Vries; Marcel Beld |
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Publication Detail:
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Type: Journal Article Date: 2011-12-05 |
Journal Detail:
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Title: Advances in virology Volume: 2011 ISSN: 1687-8647 ISO Abbreviation: Adv Virol Publication Date: 2011 |
Date Detail:
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Created Date: 2012-02-07 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101508613 Medline TA: Adv Virol Country: United States |
Other Details:
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Languages: eng Pagination: 514681 Citation Subset: - |
Affiliation:
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Royal Tropical Institute, KIT Biomedical Research, Meibergdreef 39, 1105 AZ Amsterdam, The Netherlands. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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