Document Detail


Internalization and resistance to degradation of Alzheimer's A beta 1-42 at nanomolar concentrations in THP-1 human monocytic cell line.
MedLine Citation:
PMID:  10076859     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Microglial cell involvement in Alzheimer's disease has been related to amyloid beta (A beta) internalization, the release of inflammatory cytokines and the development of neuritic plaques. The human monocyte/macrophage THP-1 cell line has been widely used as a model of human microglial cells. We used THP-1 cells to study the adsorption, internalization and resistance to degradation of A beta1-40 and A beta1-42 isoforms offered at nanomolar concentrations and free of large aggregates, conditions that may mimic a pre-fibrillar stage of A beta in the brain. Under these conditions, A betas did not induce THP-1 activation, as assessed by interleukin-1beta expression. A beta1-42 showed a preferential adsorption and intracellular accumulation as compared to A beta1-40, supporting that competent nuclei for A beta1-42 ordered aggregation may be formed inside microglial cells. In light of the possible neurotoxicity of soluble A beta1-42, we propose that amyloid formation within brain phagocytic cells may be a protective mechanism in early stages of the disease.
Authors:
L Morelli; G H Giambartolomei; M I Prat; E M Castaño
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Neuroscience letters     Volume:  262     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  1999 Feb 
Date Detail:
Created Date:  1999-05-06     Completed Date:  1999-05-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  IRELAND    
Other Details:
Languages:  eng     Pagination:  5-8     Citation Subset:  IM    
Affiliation:
Instituto de Química y Fisicoquímica Biológicas (IQUIFIB) and Departamento de Química Biológica, Facultad de Farmacia y Bioquímica, Universidad de Buenos Aires, Argentina.
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MeSH Terms
Descriptor/Qualifier:
Amyloid beta-Protein / metabolism*
Cell Line
Humans
Microglia / metabolism
Monocytes / metabolism*
Peptide Fragments / metabolism*
Senile Plaques / metabolism
Chemical
Reg. No./Substance:
0/Amyloid beta-Protein; 0/Peptide Fragments; 0/amyloid beta-protein (1-42)

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