| Internal intensity standards for heme protein UV resonance Raman studies: excitation profiles of cacodylic acid and sodium selenate. | |
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MedLine Citation:
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PMID: 1846749 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We examine the utility of SO4(2-), ClO4-, cacodylic acid, and SeO4(2-) as internal intensity standards for Raman spectral measurements of protein structure. We find that 0.1 M SO4(2-) and ClO4- perturb the protein tertiary structure of aquomethemoglobin (met-Hb) and its fluoride (met-HbF) and azide (met-HbN3) complexes. Changes occur for the tryptophan near-UV absorption bands, the iron spin state is altered, and the fluoride ligand affinity decreases. Concentrations of ClO4- and SO4(2-) as low as 0.1 M suppress the met-HbF quaternary R----T transition induced by the allosteric effector inositol hexaphosphate (IHP). In contrast, similar concentrations of cacodylic acid and SeO4(2-) show little effect on the hemoglobin tertiary or quaternary protein structures or upon the R----T transition induced by IHP. We measure the Raman cross sections of cacodylic acid and SeO4(2-) between 218 and 514.5 nm and find that for UV excitation they are ca. 5-fold larger than ClO4- or SO4(2-). Thus, cacodylic acid and selenate can be used at lower concentrations. Cacodylic acid and SeO4(2-) are superior Raman internal intensity standards for protein structural studies. |
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Authors:
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S H Song; S A Asher |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Biochemistry Volume: 30 ISSN: 0006-2960 ISO Abbreviation: Biochemistry Publication Date: 1991 Feb |
Date Detail:
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Created Date: 1991-03-14 Completed Date: 1991-03-14 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0370623 Medline TA: Biochemistry Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1199-205 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of Pittsburgh, Pennsylvania 15260. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cacodylic Acid
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chemistry Humans Iron / chemistry Methemoglobin / chemistry* Perchloric Acid Selenium / chemistry Selenium Compounds* Spectrophotometry, Ultraviolet Spectrum Analysis, Raman / methods* Sulfates / chemistry |
| Grant Support | |
ID/Acronym/Agency:
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IR01GM30741-09/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Selenium Compounds; 0/Sulfates; 13410-01-0/sodium selenate; 7439-89-6/Iron; 75-60-5/Cacodylic Acid; 7601-90-3/Perchloric Acid; 7782-49-2/Selenium; 9008-37-1/Methemoglobin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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