| Intermittent capillary perfusion in rat pancreas grafts following short- and long-term preservation in University of Wisconsin solution. | |
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MedLine Citation:
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PMID: 16573549 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In pancreas transplantation (PTx), ischemia/reperfusion-induced deterioration of graft-microcirculation is accompanied by alterations of intermittent capillary perfusion (IP; alternating cessation and resumption of capillary blood flow) is known to counteract malperfusion. Incidence and effectiveness of IP following short- versus long-term preservation of pancreas grafts with University of Wisconsin (UW) solution has not been examined so far. PTx was performed in Lewis rats following 2-h or 18-h preservation in UW solution. Using intravital fluorescence microscopy, functional capillary density (FCD), red blood cell (RBC) velocity, IP-incidence and -frequency were analyzed. Laser Doppler flowmetry allowed for the determination of erythrocyte flux and velocity. Measurements were performed at 30, 60 and 120 min after reperfusion. Nontransplanted animals served as controls. FCD, RBC-velocity and -flux remained unchanged in the 2-h group. IP was encountered in 87% of all observation areas at 120 min. After 18-h ischemia, FCD was significantly reduced, which was paralleled by a 50% incidence of IP at 120 min. Tissue edema and leukocyte infiltration in pancreas grafts following 18-h preservation were significantly enhanced. Therefore, IP is an important mechanism aimed at improving microcirculation and UW solution is suitable to preserve vasomotion-activities enabling long-term preservation in a pancreas graft. |
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Authors:
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Gerhard Preissler; Steffen Massberg; Helmut Waldner; Konrad Messmer |
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Publication Detail:
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Type: In Vitro; Journal Article |
Journal Detail:
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Title: Transplant international : official journal of the European Society for Organ Transplantation Volume: 19 ISSN: 0934-0874 ISO Abbreviation: Transpl. Int. Publication Date: 2006 Apr |
Date Detail:
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Created Date: 2006-03-31 Completed Date: 2006-06-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 8908516 Medline TA: Transpl Int Country: Germany |
Other Details:
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Languages: eng Pagination: 325-32 Citation Subset: IM |
Affiliation:
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Department of Surgery, Klinikum Grosshadern, Ludwig-Maximilians-University of Munich, Germany. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Allopurinol Animals Blood Flow Velocity Capillaries / physiology Glutathione Insulin Male Organ Preservation / methods* Organ Preservation Solutions* Pancreas / blood supply*, injuries, pathology Pancreas Transplantation Perfusion Raffinose Rats Rats, Inbred Lew Reperfusion Injury / prevention & control Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Organ Preservation Solutions; 0/University of Wisconsin-lactobionate solution; 11061-68-0/Insulin; 315-30-0/Allopurinol; 512-69-6/Raffinose; 58-61-7/Adenosine; 70-18-8/Glutathione |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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