Document Detail


Intermedin 17-47 does not function as a full intermedin antagonist within the central nervous system or pituitary.
MedLine Citation:
PMID:  17945397     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
A fragment of intermedin (IMD), IMD17-47, has been shown to antagonize the hypotensive effects of intravenous IMD administration; however, the effects of IMD17-47 have not been studied in other systems such as brain and pituitary gland. IMD17-47 was administered intracerebroventricularly (i.c.v.) into male rats alone or prior to administration of IMD; and blood pressure and food and water intakes measured. Multiple doses of IMD17-47 failed to alter basal blood pressure and heart rate, but did partially reverse the stimulatory effects of IMD given i.c.v. on blood pressure and heart rate. A low dose of IMD17-47 by itself significantly increased basal food and water intake. However, a higher dose of the antagonist did not alter food or water intake compared to control treated rats. No dose of IMD17-47 was able to reverse the inhibitory effects of IMD administered i.c.v. on food and water intake. Furthermore, IMD17-47 failed to significantly alter the inhibitory effects of IMD on growth hormone releasing hormone-stimulated growth hormone release from dispersed anterior pituitary cells in culture. A siRNA molecule designed to compromise IMD production was able to reduce brain IMD levels and did, upon i.c.v. administration, cause increased water drinking in male rats. This tool may provide a better method than the use of the IMD17-47 compound to study the role of endogenous IMD within the CNS and pituitary.
Authors:
Meghan M White; Willis K Samson
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural     Date:  2007-09-11
Journal Detail:
Title:  Peptides     Volume:  28     ISSN:  0196-9781     ISO Abbreviation:  Peptides     Publication Date:  2007 Nov 
Date Detail:
Created Date:  2007-10-29     Completed Date:  2008-02-26     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8008690     Medline TA:  Peptides     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2171-8     Citation Subset:  IM    
Affiliation:
Saint Louis University, School of Medicine, Department of Pharmacological and Physiological Science, 1402 S. Grand Boulevard, St. Louis, MO 63104, USA. mwhite44@slu.edu
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MeSH Terms
Descriptor/Qualifier:
Adrenomedullin / chemistry,  genetics,  pharmacology*
Animals
Blood Pressure / drug effects
Cell Line
Central Nervous System / cytology,  drug effects*,  metabolism
Drinking / drug effects
Eating / drug effects
Growth Hormone / metabolism
Heart Rate / drug effects
Humans
Injections, Intraventricular
Male
Neuropeptides / chemistry,  genetics,  pharmacology*
Peptide Fragments / administration & dosage,  chemistry,  pharmacology*
Pituitary Gland / cytology,  drug effects*,  metabolism
RNA Interference
Rats
Rats, Sprague-Dawley
Grant Support
ID/Acronym/Agency:
HL 66023/HL/NHLBI NIH HHS
Chemical
Reg. No./Substance:
0/Neuropeptides; 0/Peptide Fragments; 0/intermedin protein, rat; 148498-78-6/Adrenomedullin; 9002-72-6/Growth Hormone

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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