| Intermediates in the degradation of mRNA from the lactose operon of Escherichia coli. | |
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MedLine Citation:
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PMID: 1710346 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have analyzed the processing of mRNA from the lac operon in an Escherichia coli strain carrying the lac on a multicopy plasmid. Messenger RNA was analyzed by hybridization and nuclease protection of pulse-labeled RNA and precursor-product relationships were determined by quantitating radioactivity in primary and processed transcripts at various times after induction of the lac promoter or inhibition of transcription with rifampicin. Our results support the existence of two types of processed transcripts with endpoints in the lacZ-lacY intercistronic region. One of these carries lacZ sequences and has a 3' endpoint about 30 bases downstream of this gene. The other carries lacY sequences and has a 5' end in the translation termination region of the lacZ gene. Finally, we have found evidence that transcription is continued at least 268 bases beyond the last gene (lacA) and that this 3' non-translated region is shortened by post-transcriptional processing. |
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Authors:
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J R McCormick; J M Zengel; L Lindahl |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Nucleic acids research Volume: 19 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1991 May |
Date Detail:
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Created Date: 1991-07-11 Completed Date: 1991-07-11 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 2767-76 Citation Subset: IM |
Affiliation:
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Department of Biology, University of Rochester, NY 14627. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Base Sequence DNA, Bacterial Escherichia coli / genetics* Kinetics Lac Operon / genetics* Molecular Sequence Data Nucleic Acid Conformation RNA, Bacterial / metabolism RNA, Messenger / metabolism* |
| Chemical | |
Reg. No./Substance:
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0/DNA, Bacterial; 0/RNA, Bacterial; 0/RNA, Messenger |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Day: 25 Month: 5 Year: 1991 Volume: 19 Issue: 10 First Page: 2767 Last Page: 2776 ID: 328199 PubMed Id: 1710346 |
| Intermediates in the degradation of mRNA from the lactose operon of Escherichia coli. | |
| J R McCormick | |
| J M Zengel | |
| L Lindahl | |
| Department of Biology, University of Rochester, NY 14627. |
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