Document Detail

Intermediate zone cells express calcium-permeable AMPA receptors and establish close contact with growing axons.
MedLine Citation:
PMID:  10632599     Owner:  NLM     Status:  MEDLINE    
Recent studies have shown that cells in the intermediate zone (IZ) of the embryonic neocortex originate in the basal telencephalon and migrate tangentially in the cortical wall (;; ). We had previously observed growing cortical axons closely apposed to calbindin-positive, tangentially oriented cells in the IZ (), and it has been shown that neurites in the IZ express a glutamate transporter (). To test if glutamate released by corticofugal growth cones could influence the tangential IZ cells, we characterized the glutamate receptors expressed by IZ cells using patch-clamp techniques, histochemical labeling, and immunostaining on slices of embryonic mice forebrain. We show that tangential IZ cells express inwardly rectifying kainate responses, but not NMDA responses, and accumulate cobalt after AMPA receptor activation. We conclude that IZ cells express calcium-permeable AMPA receptors. This property correlates with our observation that the GluR2 subunit is not expressed in the IZ. AMPA receptors are activated by a millimolar concentration of glutamate. To know whether this high level of glutamate could occur at the surface of IZ cells, we examined contacts made by corticofugal growth cones and calbindin-positive IZ cells using electron microscopy. We show vesicle-containing neurites tightly apposed to calbindin-positive IZ cells over remarkably long length. This suggests that glutamate released by growing corticofugal axons could reach high concentrations close to AMPA receptors of tangential IZ cells and efficiently activate them to control the intracellular calcium in embryonic IZ cells.
C Métin; J P Denizot; N Ropert
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  The Journal of neuroscience : the official journal of the Society for Neuroscience     Volume:  20     ISSN:  1529-2401     ISO Abbreviation:  J. Neurosci.     Publication Date:  2000 Jan 
Date Detail:
Created Date:  2000-02-09     Completed Date:  2000-02-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8102140     Medline TA:  J Neurosci     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  696-708     Citation Subset:  IM    
Equipe Régionalisation Nerveuse, Centre National de la Recherche Scientifique (CNRS) Unité Mixte de Recherche 8542, Niveau 8, Ecole Normale Supérieure, 75230 Paris Cédex 05, France.
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MeSH Terms
Action Potentials / drug effects,  physiology
Axons / drug effects,  physiology*,  ultrastructure
Benzodiazepines / pharmacology
Biological Transport / drug effects
Calcium / metabolism*
Calcium-Binding Protein, Vitamin D-Dependent / analysis
Cerebral Cortex / cytology,  physiology
Cobalt / pharmacokinetics,  pharmacology
Excitatory Amino Acid Antagonists / pharmacology
Gestational Age
Kainic Acid / pharmacology
Mice, Inbred C57BL
Mice, Inbred Strains
N-Methylaspartate / pharmacology
Neurites / physiology,  ultrastructure
Neurons / cytology,  drug effects,  physiology*
Prosencephalon / cytology,  embryology,  physiology*
Quinoxalines / pharmacology
Receptors, AMPA / metabolism*
Tetrodotoxin / pharmacology
gamma-Aminobutyric Acid / pharmacology
Reg. No./Substance:
0/Calcium-Binding Protein, Vitamin D-Dependent; 0/Excitatory Amino Acid Antagonists; 0/GYKI 53784; 0/Quinoxalines; 0/Receptors, AMPA; 0/calbindin; 12794-10-4/Benzodiazepines; 2379-57-9/FG 9041; 4368-28-9/Tetrodotoxin; 487-79-6/Kainic Acid; 56-12-2/gamma-Aminobutyric Acid; 6384-92-5/N-Methylaspartate; 7440-48-4/Cobalt; 7440-70-2/Calcium

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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