Document Detail


Intermediate filaments are required for C. elegans epidermal elongation.
MedLine Citation:
PMID:  14975728     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Cytoplasmic intermediate filaments (cIFs) are thought to provide mechanical strength to vertebrate cells; however, their function in invertebrates has been largely unexplored. The Caenorhabditis elegans genome encodes multiple cIFs. The C. elegans ifb-1 locus encodes two cIF isoforms, IFB-1A and IFB-1B, that differ in their head domains. We show that both IFB-1 isoforms are expressed in epidermal cells, within which they are localized to muscle-epidermal attachment structures. Reduction in IFB-1A function by mutation or RNA interference (RNAi) causes epidermal fragility, abnormal epidermal morphogenesis, and muscle detachment, consistent with IFB-1A providing mechanical strength to epidermal attachment structures. Reduction in IFB-1B function causes morphogenetic defects and defective outgrowth of the excretory cell. Reduction in function of both IFB-1 isoforms results in embryonic arrest due to muscle detachment and failure in epidermal cell elongation at the 2-fold stage. Two other cIFs, IFA-2 and IFA-3, are expressed in epidermal cells. We show that loss of function in IFA-3 results in defects in morphogenesis indistinguishable from those of embryos lacking ifb-1. In contrast, IFA-2 is not required for embryonic morphogenesis. Our data indicate that IFB-1 and IFA-3 are likely the major cIF isoforms in embryonic epidermal attachment structures.
Authors:
Wei-Meng Woo; Alexandr Goncharov; Yishi Jin; Andrew D Chisholm
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Developmental biology     Volume:  267     ISSN:  0012-1606     ISO Abbreviation:  Dev. Biol.     Publication Date:  2004 Mar 
Date Detail:
Created Date:  2004-02-20     Completed Date:  2004-06-15     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  0372762     Medline TA:  Dev Biol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  216-29     Citation Subset:  IM    
Affiliation:
Sinsheimer Laboratories, Department of Molecular, Cellular, and Developmental Biology, University of California, Santa Cruz, CA 95064, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Blotting, Western
Caenorhabditis elegans / embryology*,  genetics
Epidermis / embryology*,  ultrastructure
Microscopy, Electron
Morphogenesis
RNA Interference
Grant Support
ID/Acronym/Agency:
GM54657/GM/NIGMS NIH HHS; T32-GM08646/GM/NIGMS NIH HHS

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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