Document Detail

Intermediate basal cells of the prostate: in vitro and in vivo characterization.
MedLine Citation:
PMID:  12692787     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Progenitor cells within the prostate basal layer may play important roles in differentiation and carcinogenesis; however, prostate stem cell populations remain uncharacterized. METHODS: Immunohistochemical and immunoblot analyses were used to characterize prostate epithelial cells (PrEC), a commercially available prostate basal cell isolate. RESULTS: Proliferating PrECs exhibited immunophenotypic characteristics most consistent with basal cells, but during senescence PrECs up-regulated androgen receptor (AR) mRNA, p27, and low-molecular-weight cytokeratin (LMWCK) expression, suggestive of partial differentiation. PrECs also stained strongly for involucrin, which marked a subset of intermediate prostate basal cells in vivo. Basal hyperplasia consisting of involucrin-positive cells was prevalent in prostate tissue from androgen-ablated patients, and formed epithelial clusters flanked by involucrin-negative basal and luminal monolayers. Cultivation of PrECs on matrigel together with androgen-treated stromal conditioned media resulted in dense aggregates, with a peripheral rim of basal-like cells expressing p63 and basal cytokeratins. CONCLUSIONS: PrEC represents an epithelial population whose basal characteristics are modified in response to matrigel, stromal factors, and senescence, consistent with a transient amplifying population. These cells may derive from a previously unrecognized, involucrin-positive subset present in vivo.
Levi A Garraway; Douglas Lin; Sabina Signoretti; David Waltregny; James Dilks; Nandita Bhattacharya; Massimo Loda
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Prostate     Volume:  55     ISSN:  0270-4137     ISO Abbreviation:  Prostate     Publication Date:  2003 May 
Date Detail:
Created Date:  2003-04-14     Completed Date:  2003-05-23     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  8101368     Medline TA:  Prostate     Country:  United States    
Other Details:
Languages:  eng     Pagination:  206-18     Citation Subset:  IM    
Copyright Information:
Copyright 2003 Wiley-Liss, Inc.
Department of Medical Oncology, Dana Farber Cancer Institute, Harvard Medical School, Boston, Massachusetts 02115, USA.
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MeSH Terms
Blotting, Western
Cell Aging / physiology
Cell Differentiation
Epithelial Cells / cytology,  metabolism
Keratins / metabolism
Prostate / cytology*,  metabolism
Prostatic Neoplasms / metabolism,  pathology
Protein Precursors / metabolism
RNA / chemistry,  genetics
Receptors, Androgen / genetics,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells / cytology,  metabolism
Grant Support
Reg. No./Substance:
0/Protein Precursors; 0/Receptors, Androgen; 60108-77-2/involucrin; 63231-63-0/RNA; 68238-35-7/Keratins

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