Document Detail


Interleukin (IL)-6 and IL-1 beta act synergistically within the brain to induce sickness behavior and fever in rats.
MedLine Citation:
PMID:  18255258     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Pro-inflammatory cytokines interleukin (IL)-6 and IL-1 beta can act in the brain (centrally) to cause fever. Sickness behaviors which accompany fever also appear to involve the central action of IL-1 beta. We injected species-homologous rat IL-6 and IL-1 beta directly into the brains of conscious rats to examine the effect of these cytokines on fever, and two behaviors affected by sickness, voluntary wheel-running and food intake. Male Sprague-Dawley rats selected for their predisposition to spontaneously run on running wheels were used in the experiment. Each rat was anaesthetized and had a temperature-sensitive radiotransmitter implanted intra-abdominally, and a 23-gauge stainless steel guide cannula inserted stereotaxically over the lateral cerebral ventricle. Rats were randomly assigned to receive intracerebroventricular injections of three doses of either IL-1 beta or IL-6 (100 ng, 1 ng or 0.1 ng IL-1 beta and 200 ng, 20 ng or 2 ng IL-6), or one of three different combinations of IL-1 beta and IL-6. Rats receiving either IL-1 beta or IL-6 showed a dose-dependent increase in body temperature and decrease in wheel-running (ANOVA, p<0.0001). Only rats receiving the highest dose of IL-1 beta significantly decreased food intake and body mass compared to rats receiving vehicle (ANOVA, p<0.001). Doses of IL-1 beta and IL-6 which, when injected on their own were non-pyrogenic and did not affect food intake and body mass, induced fever and anorexia when they were co-injected centrally. These results show that species-homologous rat IL-6 and IL-1 beta can act directly within the brain to decrease voluntary activity and suggest they also can act synergistically to induce anorexia and fever.
Authors:
Lois M Harden; Irné du Plessis; Stephen Poole; Helen P Laburn
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-02-05
Journal Detail:
Title:  Brain, behavior, and immunity     Volume:  22     ISSN:  1090-2139     ISO Abbreviation:  Brain Behav. Immun.     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-07-08     Completed Date:  2008-08-12     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8800478     Medline TA:  Brain Behav Immun     Country:  United States    
Other Details:
Languages:  eng     Pagination:  838-49     Citation Subset:  IM    
Affiliation:
Brain Function Research Group, School of Physiology, Medical School, University of the Witwatersrand, 7 York Road, Parktown, Gauteng, Johannesburg, South Africa. Lois.Harden@wits.ac.za
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MeSH Terms
Descriptor/Qualifier:
Analysis of Variance
Animals
Anorexia / chemically induced,  physiopathology
Behavior, Animal / drug effects*
Body Temperature / drug effects
Body Weight / drug effects
Brain / drug effects*,  physiopathology
Dose-Response Relationship, Drug
Drug Synergism
Eating / drug effects
Fever / chemically induced*
Injections, Intraventricular
Interleukin-1beta / administration & dosage,  toxicity*
Interleukin-6 / administration & dosage,  toxicity*
Male
Motor Activity / drug effects
Rats
Rats, Sprague-Dawley
Sick Role
Chemical
Reg. No./Substance:
0/Interleukin-1beta; 0/Interleukin-6

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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